| Japanese |
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| Title | 99mTc標識抗体F (ab') 2分画を用いる卵巣癌画像診断の基礎的研究 |
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| Subtitle | 原著 |
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| Authors | 杉山純夫* |
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| Organization | *群馬大学医学部放射線医学教室 |
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| Journal | 核医学 |
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| Volume | 31 |
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| Number | 4 |
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| Page | 327-336 |
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| Year/Month | 1994/4 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」 卵巣癌関連抗原CA125を認識するモノクローナル抗体OC125のF (ab') 2分画を99mTc標識し, 99mTc標識抗体を用いる卵巣癌画像診断の基礎的検討を放射性ヨード標識抗体と比較しながら行った. 99mTc標識率は99.9%と著しく良好で, コロイド形成も認められなかった. 細胞表面にCA125を発現するPC-9細胞107個への結合率は47.0%で, 125I標識抗体とほぼ同様に抗体活性は保持されていた. PC-9細胞を移植したヌードマウスでの体内分布で, 投与18時間後の腫瘍/血液比は5.73±1.41と, 125I標識抗体投与群の3.63±0.27に比べ有意に高かった (P<0.01) . シンチグラムでも良好な腫瘍描出が得られ, 99mTc標識OC125抗体F (ab') 2分画の卵巣癌画像診断への臨床応用が期待される. |
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| Practice | 臨床医学：一般 |
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| Keywords | Ovarian cancer, CA 125, Radioimmunodetection, OC125, 99mTc. |
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| English |
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| Title | Basic Study for Immunoscintigraphy of Ovarian Cancer Using 99mTc Labeled F (ab') 2 Fragments of Monoclonal Antibody |
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| Subtitle | Original Articles |
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| Authors | Sumio SUGIYAMA |
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| Authors(kana) | |
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| Organization | Department of Radiology, Gunma University School of Medicine |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 31 |
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| Number | 4 |
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| Page | 327-336 |
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| Year/Month | 1994/4 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] Basic study was performed to evaluate the efficacy of immunoscintigraphy of ovarian cancer using 99mTc labeled F (ab') 2 fragments of monoclonal antibody OC125 which was reactive with CA125 antigen. Labeling efficiency was 99.9% and colloid formation was not observed. Specific activity was 1.48 GBq (40 mCi) /mg antibody and 99mTc OC125 preparation was stable more than 3 hr after labeling. Percent binding to 107 cells of PC-9 cancer cells expressing CA125 antigen was 47.0% in 99mTc OC125 almost the same as that of 125I OC125, Tumor-to-blood ratio for 99mTc OC125 was 5.73+-1.41 at 18 hr after intravenous injection, which was significantly higher than that for 125I OC125. The scintigram showed a good localization in nude mouse bearing PC-9 tumor cells, and the clinical use of 99mTc OC125 is promising for the imaging of ovarian cancer. |
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| Practice | Clinical medicine |
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| Keywords | Ovarian cancer, CA 125, Radioimmunodetection, OC125, 99mTc. |
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