| Japanese |
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| Title | イムノラジオメトリックアッセイによる血中βhCG測定法に関する基礎的検討 |
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| Subtitle | 技術報告 |
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| Authors | 尾森春艶*, 末廣美津子*, 福地稔* |
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| Authors(kana) | |
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| Organization | *兵庫医科大学核医学科 |
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| Journal | 核医学 |
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| Volume | 29 |
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| Number | 6 |
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| Page | 709-716 |
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| Year/Month | 1992/6 |
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| Article | 報告 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」IRMAによる血中βhCG測定法エルザ・F-βHCG・キットにつき基礎的検討を行った. 本法の最小検出感度は0.05ng/mlで, インキュベーション時間および温度を変えても標準曲線に対する有意の影響はなかった. 本法に対する交差率はLH 0.13%, FSH 0.02%, TSH 0.02%そしてhCG 2.5%であった. さらにエルザチューブを用いhCG中のαhCGから遊離したβhCGを吸収して同様の検討を行ったところ, 交差率は0.14%であった. 従来のβhCG RIAキットと本法との測定値の比較では, 両者はよく相関(r=+0.961)したが, 絶対値は, 本法で低値(y=0.35x+0.26)であった. その他, 希釈試験, 回収試験, 精度再現性の結果も良好であることから, B., F. 分離を必要としない簡便な本法は, よりβhCGに特異的な測定法として臨床的に有用と考えられた. |
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| Practice | 臨床医学：一般 |
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| Keywords | βhCG, IRMA, Monoclonal antibody. |
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| English |
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| Title | Fundamental Evaluation of ELSA・F-βHCG Kit as an Immunoradiometric Assay Specific for Serum βHCG |
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| Subtitle | Technical Reports |
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| Authors | Shunen OMORI, Mitsuko SUEHIRO, Minoru FUKUCHI |
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| Authors(kana) | |
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| Organization | Department of Nuclear Medicine, Hyogo College of Medicine |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 29 |
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| Number | 6 |
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| Page | 709-716 |
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| Year/Month | 1992/6 |
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| Article | Report |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary]We evaluated ELSA・F-βHCG kit as an immunoradiometric assay (IRMA) specific for serum β human chorionic gonadotropin (βhCG). This IRMA was found to be highly sensitive to serum βhCG; the minimum detectable concentration of βhCG was 0.05 ng/ml. No significant effects on the standard curves were observed when first and second incubation time and temperature were varied from 30 min to 240 min and from 4℃ to 37℃, respectively. Commercial LH, FSH, and TSH had little effect on the assay system; the cross-reactivity of commercial hCG was 2.5%, and 0.14% after unconjugated βhCG with α-subunit was absorbed with ELSA-tube. Multiple dilutions of sera of pregnancy resulted in curves paralleling that obtained using standard βhCG; the recovery of βhCG added to the serum was 98.1+-2.5% (mean+-SD), and mean coefficient of variation of the interassay reproducibility of serum βhCG (n=10) was 6.7+-2.8 (SD)%. Serum βhCG concentration measured using ELSA・F-βHCG kit was well correlated with that measured using conventional βhCG RIA kit (r=+0.961, p<0.01), although values were lower than those measured with the latter (y=0.35x+0.26). Our results suggest that ELSA・F-βHCG kit is a useful assay system for serum βhCG. |
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| Practice | Clinical medicine |
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| Keywords | βhCG, IRMA, Monoclonal antibody. |
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