| Japanese |
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| Title | RI標識モノクローナル抗体を用いる新しい血栓診断法に関する研究 - 第1編 ウサギならびにヒト血小板に対するモノクローナル抗体の作成 - |
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| Subtitle | 原著 |
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| Authors | 中島鉄夫*, 遠藤啓吾**, 山本和高**, 阪原晴海**, 小泉満**, 河村泰孝**, 太田仁八**, 国松美帆子**, 小西淳二**, 永田博一***, 野村昌作***, 粉川晧年***, 安永幸二郎***, 松岡洋一郎****, 鳥塚莞爾***** |
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| Authors(kana) | |
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| Organization | *福井医科大学放射線科, **京都大学医学部核医学科, ***関西医科大学第一内科, ****三重大学医学部放射線科, *****副学長 |
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| Journal | 核医学 |
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| Volume | 25 |
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| Number | 8 |
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| Page | 743-748 |
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| Year/Month | 1988/8 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」動脈血栓症をイムノシンチグラフィの手法により描出する目的で, ウサギおよびヒトの血小板に対するモノクローナル抗体を作成しその性状の検討を行った. ウサギ血小板に対して12種, ヒト血小板に対しては2種の抗体が確立された. これらはすべて血小板特異的で, 血小板の凝集能に影響を及ぼすものが多かった. これらのなかでウサギ血小板を抗原として作られた138-10は1.2×10 9M-1の高い親和定数でウサギ血小板と結合し, ヒトの血小板に対して作られた抗体1-19の親和定数は2.1×10 9M-1であったが, ウサギの血小板ともほぼ同様の親和定数で結合した. また, これら二つの抗体はともに血小板の凝集能にほとんど影響を及ぼさなかった. 138-10と1-19はその高い親和定数とウサギ血小板凝集能に影響を与えない特性から, ウサギを用いた血栓シンチグラフィの基礎検討に応用可能と思われた. |
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| Practice | 臨床医学：一般 |
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| Keywords | Thrombus scintigraphy, Immunoscintigraphy, Monoclonal antibody, Platelet. |
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| English |
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| Title | Thrombus Scintigraphy Using Radiolabeled Monoclonal Antibodies to Platelets : (I) Development of Monoclonal Antibodies Reactive with Rabbit and Human Platelets |
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| Subtitle | - Original Articles - |
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| Authors | Tetsuo NAKASHIMA*, Keigo ENDO**, Kazutaka YAMAMOTO**, Harumi SAKAHARA**, Mitsuru KOIZUMI**, Yasutaka KAWAMURA**, Hitoya OOTA**, Mihoko KUNIMATSU**, Junji KONISHI**, Hirokazu NAGATA***, Syousaku NOMURA***, Hirotoshi KOKAWA***, Koujirou YASUNAGA***, Youichiro MATSUOKA****, Kanji TORIZUKA* |
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| Authors(kana) | |
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| Organization | *Department of Radiology, Fukui Medical School, **Department of Nuclear Medicine, Kyoto University School of Medicine, ***The First Department of Internal Medicine, Kansai Medical University, ****Department of Radiology, Mie University School of Medicine |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 25 |
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| Number | 8 |
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| Page | 743-748 |
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| Year/Month | 1988/8 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | Intending to use for the immunoscintigraphy (IS) of arterial thrombosis, we have generated monoclonal antibodies reactive with rabbit and human platelets. Twelve monoclonal antibodies were established by the immunization of rabbit platelets and two by human platelets. All antibodies were specific for platelets, but some recognized both human and rabbit platelets. Antibody, designated 138-10, bound only to rabbit platelets with a high association constant of 1.2×10 9M-1 and showed little effect on the aggregation of platelets. Another antibody 1-19 was produced by the immunization of human platelets but cross-reacted with rabbit platelets. As far as we know, this is the first report describing about monoclonal antibodies reactive with rabbit platelets, and these radio-labeled monoclonal antibodies will be of great use for the study of thrombus scintigraphy. |
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| Practice | Clinical medicine |
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| Keywords | Thrombus scintigraphy, Immunoscintigraphy, Monoclonal antibody, Platelet. |
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