| Japanese |
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| Title | 125I-Cortisol, Polyethylene Glycolを用いた血漿Cortisol Radioimmunoassayに関する研究 |
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| Subtitle | 原著 |
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| Authors | 西啓子*, 荻原俊男**, 宮井潔*, 熊原雄一**, 石橋嘉一郎*** |
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| Organization | *大阪大学医学部中央臨床検査部, **第4内科, ***栄研イムノケミカル研究所 |
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| Journal | 核医学 |
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| Volume | 13 |
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| Number | 4 |
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| Page | 469-477 |
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| Year/Month | 1976/8 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「I. はじめに」1969年, Abrahamにより報告されたestradiolのradioimmunoassay(RIA)は, その後, 種々のステロイドホルモンの測定に応用され, 感度, 及び特異性の点ですぐれている為, 従来の化学的な方法にとって代りつつある. cortisolについても, 螢光法やcompetitive protein binding analysis(CPBA)法に加え, 1972年Ruderらにより, RIAが報告された. cortisol測定は, 臨床上重要な検査であり, その需要も多く, 一般検査として, 多数検体処理が必要であるが, 3H-標識ステロイドによる, RIAは操作が煩雑であり普及し難かった. その点を解決すべく, 125I-標識ステロイドが最近開発された, さらにBound-Free(B-F)分離法として, 簡便なPolyethylene glycol(PEG)法がSchillerらによりステロイドホルモンに応用されている. |
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| Practice | 臨床医学：一般 |
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| English |
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| Title | Plasma Cortisol Radioimmunoassay With 125I-cortisol and Polyethylene glycol |
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| Subtitle | Original Articles |
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| Authors | Keiko NISHI, Toshio OGIHARA, Kiyoshi MIYAI, Yuichi KUMAHARA, Kaichiro ISHIBASHI* |
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| Organization | The Central Laboratory for Clinical Investigation, Osaka University Hospital, *Eiken Immunochemical Institute |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 13 |
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| Number | 4 |
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| Page | 469-477 |
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| Year/Month | 1976/8 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] A new, convenient and less time-consuming plasma cortisol radioimmunoassay was set up. Antibody raised to cortisol-21-hemisuccinate-BSA was used at 1:6400 dilution. Because of its relatively high specificity, direct assay was possible. Main improved points were as follows, i.e., i) 125I-cortisol was used as labelled compound, ii) B-F separation was done by polyethylene glycol, iii) the effect of endogeneous corticosteroid binding globulin(CBG) was avoided by 0.6% glutamate buffer pH 3.3 treatment. It was found that the omission of heat treatment was possible to avoid this CBG effect. Minimum detectable concentration was 0.1ng/tube, and the assay range was 1 to 80μg/dl plasma when 10μl sample was used. Precision and accuracy were satisfactory. Coefficient of variance for intraassay and interassay were 6.0% at 12.1μg/dl, 3.8% at 36.6μg/dl and 7.5% at 10.2μg/dl, 6.7% at 41.8μg/dl respectively. Data obtained by this method were compared to those by Murphy's CPBA method and other commercial RIA method. They were quite comparable with each other. Mean value of serum cortisol level (8:30-11:30) in normal subjects was 7.7+-3.4μg/dl (m+-S.D., n=102). Mean value of serum cortisol was decreased slightly but significantly with age. |
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| Practice | Clinical medicine |
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