| Japanese |
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| Title | Equilibrium dialysisを用いた血中遊離型 (蛋白非結合型) cortisol動態の検討 |
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| Subtitle | 原著 |
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| Authors | 橋本浩三*, 浅岡克司**, 大藤真** |
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| Organization | *岡山大学医学部中央検査部, **岡山大学医学部第三内科 |
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| Journal | 核医学 |
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| Volume | 11 |
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| Number | 5 |
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| Page | 531-539 |
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| Year/Month | 1974/10 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「はじめに」血中cortisolは, 一部は特異的結合蛋白であるcorticosteroid binding globulin(CBG)と結合しており, 一部はalbuminと結合している. また一部はこれらの蛋白と結合せず, 遊離型(蛋白非結合型)cortisolとして存在している, 生理的活性を有するのはこの蛋白非結合型cortisol(non protein-bound cortisol)であるとされている. この遊離型cortisolを簡単な平衡透析を利用した方法で測定し, その動態につき多少の知見を得たので報告する. また合成エストローゲン製剤であるdiethylstilbestrol(DES)の遊離型cortisolの変動に及ぼす影響についても検討したので報告する. 「方法」1)血中蛋白非結合型cortisol, および蛋白非結合パーセントの測定方法はDoeらの方法を多少修飾して行った. |
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| Practice | 臨床医学：一般 |
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| English |
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| Title | Studies on Non Protein - Bound Cortisol with Equilibrium Dialysis |
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| Subtitle | Original |
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| Authors | Kozo HASHIMOTO1), Katsushi ASAOKA, Tadashi OFUJI2) |
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| Organization | 1)Department of Clinical Laboratory, Okayama University Hospital, 2)The Third Department of Internal Medicine, School of Medicine, Okayama University |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 11 |
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| Number | 5 |
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| Page | 531-539 |
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| Year/Month | 1974/10 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | Dynamics of non protein-bound cortisol were investigated with simple equilibrium dialysis. One ml plasma was pipetted into a Visking tube with a diameter of 8/32"and dialyzed to equilibrium in a glass tube containing 1.5 ml phosphate buffer at 37℃for 16 hours. Tracer dose of 14C-cortisol(0.002μCi, 0.014μg)was added inside or outside a cellophane tube. Following results were obtained. 1)Same percentage of unbound cortisol was obtained regardless of wheather 14C-cortisol was added inside or outside the tube. 2)Unbound percentage of plasma cortisol in 14 normal subjects was 6.6+-2.2(SD)% and non protein-bound cortisol was 0.63+-0.28(SD)μg/dl. 3)Non protein-bound cortisol was increased after injection ofα1-24ACTH, decreased after administration of dexamethasone, increased in patients with Cushing's syndrome and decreased in patients with adrenal insufficiency. 4)In patients treated with 7.5 mg diethylstilbestrol(DES: synthetic estrogen)for 3 days, plasma total cortisol was higher than in control group, whereas unbound percentage was decreased. 5)Diurnal variation of unbound percentage of cortisol was not found, but similar variation to total cortisol was found in non protein-bound cortisol. 6)In rapid ACTH test, increase of total cortisol was greater in DES treated group than in control group. Non protein-bound cortisol was also increased in DES treated group. Increase of corticosteroid binding globulin-bound cortisol(CBG)was most significant, although albumin-bound cortisol was also increased in DES treated group. So that, not only increase of CBG but any other factors appear to be concerned in significant increase of total cortisol at rapid ACTH test in DES treated group. |
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| Practice | Clinical medicine |
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