| Japanese |
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| Title | Radioimmunoassayを用いる血中1,25-dihydroxyvitamin D測定法 |
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| Subtitle | 《技術報告》 |
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| Authors | 山本逸雄*, 浜津尚就*, 木上裕輔*, 森田陸司* |
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| Authors(kana) | |
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| Organization | *滋賀医科大学放射線科・放射線部 |
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| Journal | 核医学 |
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| Volume | 34 |
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| Number | 4 |
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| Page | 243-250 |
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| Year/Month | 1997/4 |
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| Article | 報告 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」RIAを用いる血中1,25-dihydroxyvitamin Dの新しい測定キットの基礎的および臨床的検討を行った. 再現性, 回収率, 他のビタミンD代謝物との交叉等は問題なく, また従来の測定法との相関も良好であった. 本法によって測定した111例の19-78歳健常者の血中レベルの平均値は40.5±14. 2pg/mlであった. また未治療の腎不全症例や副甲状腺機能低下症患者において低値を示し, 一方, 原発性副甲状腺機能亢進症患者や, サルコイドーシスに伴う高カルシウム血症患者において, 高値を示した. 本測定法は, 従来の方法と異なり, β線を用いないので扱いが容易であり, 臨床的に有用と考えられた. 「I. はじめに」1,25-dihydroxyvitamin D(1,25-(OH)2 D)は, 腸管からのカルシウム吸収や破骨細胞形成などカルシウム代謝調節の重要なホルモンであり, コレステロールや食事中のエルゴステロールより皮膚にて紫外線によってビタミンDとなり, 肝, 腎での水酸化を経て形成される. |
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| Practice | 臨床医学：一般 |
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| Keywords | 1,25-dihydroxyvitamin D, Radioimmunoassay, Calcium metabolism, Radioreceptor assay. |
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| English |
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| Title | Measurement of Circulating 1,25-Dihydroxyvitamin D Employing Radioimmunoassay |
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| Subtitle | |
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| Authors | Itsuo YAMAMOTO, Hisatoshi HAMAZU, Yuusuke KIGAMI, Rikushi MORITA |
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| Authors(kana) | |
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| Organization | Department of Radiology and Isotope Division, Shiga University of Medical Science |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 34 |
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| Number | 4 |
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| Page | 243-250 |
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| Year/Month | 1997/4 |
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| Article | Report |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | Measurement of serum 1,25-dihydroxyvitamin D levels is important for diagnosis of various calcium metabolism disorders. Conventional assays for 1,25-dihydroxyvitamin D employed specific 1,25-dihydroxyvitamin D receptor as binding site for the ligand and thus, biologically active 1,25-dihydroxyvitamin D ligand, which is labeled with 3H, was required. Usage of 3H made assays cumbersome works. A new assay which uses specific antibody as the binding site and the radioligand labeled with 125I is now available as a commercial kit. Using these kits, we first studied basically the reproducibility, recovery, cross-reactivity and comparison with conventional assays. All of those results were satisfactory. Secondly, we measured clinically in 111 healthy adults and in patients with various disorders such as renal failure, primary hyperparathyroidism, hypoparathyroidism and sarcoidosis. This newly available kit for measurement of circulating 1,25-dihydroxyvitamin D is proved to be useful in clinical evaluation of calcium metabolic disorders. |
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| Practice | Clinical medicine |
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| Keywords | 1,25-dihydroxyvitamin D, Radioimmunoassay, Calcium metabolism, Radioreceptor assay. |
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