| Japanese |
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| Title | レーザ分光法を用いた同位体分析計と13C - 呼気検査応用 |
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| Subtitle | 技術報告 |
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| Authors | 東陽二*, 大原裕康**, 成木行彦***, 井上優介****, 佐々木康人**** |
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| Organization | *日本無線(株)研究所, **聖マリアンナ医科大学第三内科, ***東邦大学医学部第一内科, ****東京大学医学部放射線科 |
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| Journal | 核医学 |
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| Volume | 33 |
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| Number | 4 |
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| Page | 415-421 |
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| Year/Month | 1996/4 |
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| Article | 報告 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」従来から13C-呼気検査用同位体分析計として質量分析計(MS)が使われているが, 測定に熟練を要す, 高価である等の理由から, 臨床での使用の制約となっている. 臨床において, 簡便に使用できかつ信頼性の高い分析計として, レーザ分光法を用いた同位体分析計を開発し, 種々の13C-呼気検査にて, その適用性を評価した. 同一試料の繰り返し測定値の変動は0.65‰(1σ)以下であり, また, 13C-グリシン, 13C-メタセチン, 13C-尿素化合物を経口投与した呼気検査を行い, MSと比較した結果, 非常によく一致し, 有効な分析装置と言える. 「I. はじめに」炭素の安定同位体13Cで標識した化合物を経口投与後, 呼気中に排出された13CO2を分析し, 消化・吸収・代謝機能等の疾患を診断する13C-呼気検査は放射線被曝を伴わずかつ無侵襲な検査方法なので, 成人はもちろん, 乳幼児, 妊婦, 若年成人等広い範囲に適用できることが特徴である. |
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| Practice | 臨床医学：一般 |
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| Keywords | Laser spectroscopy, 13C-breath test, Stable isotope analyzer. |
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| English |
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| Title | Application of Laser Spectroscopy for 13C - Breath Tests |
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| Subtitle | Technical Reports |
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| Authors | Yoji HIGASHI*, Hiroyasu OHARA**, Yukihiko NARUKI***, Yusuke INOUE****, Yasuhito SASAKI**** |
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| Organization | *Laboratory, Japan Radio Co., Ltd., **Third Department of Internal Medicine, St. Marianna University School of Medicine, ***First Department of Internal Medicine, Toho University School of Medicine, ****Department of Radiology, Faculty of Medicine, University of Tokyo |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 33 |
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| Number | 4 |
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| Page | 415-421 |
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| Year/Month | 1996/4 |
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| Article | Report |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary]A new device Tunable Diode Laser Spectroscopy(LS)was developed for the analysis of isotope ratios of 13CO2/12CO2. Its applicability for breath tests was validated. The exhaled breath is collected in a 2l aluminum bag, of which CO2 is separated by cryogenic system and introduced into LS. Repeat measurements(8 times)of two kinds of gasses(δ13C : -2.62 and -1.14%) revealed 0.045% and 0.065% variation(1S. D. ), from which precision of measurement was estimated as 0.2% (3S. D. ). Seven healthy volunteers were given orally different dose of 13C-glycine(50 mg-200 mg in 5 subjects) and 13C methacetin(30 mg and 150 mg in 2 subjects). One patient with gastric ulcer with helicobacter pylori(HP) infection was given 100 mg of 13C-urea. Serial breath samples(9-15) were taken and measured both by LS and mass spectrometer(MS) thereafter. Appreciable peaks were obtained at 30-50 min. after glycine and methacetin ingestions. The height of the peaks were dose dependent. Increased excretion of 13CO2 characteristics to HP infection was obtained in 13C-urea breath test. Measured values with LS were identical with those measured with MS(60 samples) with the range of difference within 0.2%(3S. D. ). [Y(LS)=1.02X(MS)+0.55, r=0.996]We conclude the LS is suitable for various 13C-breath tests. |
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| Practice | Clinical medicine |
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| Keywords | Laser spectroscopy, 13C-breath test, Stable isotope analyzer. |
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