| Japanese |
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| Title | TRAb 「デイド」 キットを用いた甲状腺患者血中TSHレセプター抗体価の測定 |
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| Subtitle | 技術報告 |
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| Authors | カリル アーメド*, 冨吉勝美*, 深沢和浩**, 井上登美夫*, 村上正己***, 小林功****, 遠藤啓吾* |
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| Authors(kana) | |
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| Organization | *群馬大学医学部核医学科, **多野総合病院, ***群馬大学医学部第一内科, ****臨床検査医学 |
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| Journal | 核医学 |
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| Volume | 32 |
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| Number | 7 |
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| Page | 695-701 |
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| Year/Month | 1995/7 |
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| Article | 報告 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」バセドウ病は患者血中に存在するTSHレセプター抗体(TRAb)により発症すると考えられている. TRAb測定用キットTRAb「デイド」を用いて甲状腺疾患患者血中のTRAb測定の基礎的, 臨床的検討を行った. 本キットの測定原理は125I-標識TSHの甲状腺レセプターへの結合阻害程度を検出するものである. アッセイの温度, 時間はTRAb値にほとんど影響せず, 測定の再現性も良好であった. またビリルビン, ヘモグロビン, 乳ビも影響しなかった. カットオフ値を15%以下とすると, 55例の健常人は全例陰性に, 68例の未治療バセドウ病患者は全例陽性であったが, 治療後には陽性率が22.4%に低下した. 慢性甲状腺炎, 亜急性甲状腺炎, 慢性腎不全患者もTRAbはほとんど陰性であった. すでに市販されているTRAb「コスミック」により測定したTRAbときわめてよく一致し, 本キットによる血中TRAb値の測定は甲状腺疾患の診療に有用と考えられた. |
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| Practice | 臨床医学：一般 |
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| Keywords | TSH receptor antibodies, Radioreceptor assay, 125I labeled TSH, Graves' disease. |
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| English |
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| Title | Measurement of Serum TSH Receptor Antibodies in Thyroid Patients Using TRAb Dade Kits |
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| Subtitle | |
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| Authors | Khalil AHMED*, Katsumi TOMIYOSHI*, Kazuhiro FUKAZAWA**, Tomio INOUE*, Masami MURAKAMI***, Isao KOBAYASHI****, Keigo ENDO* |
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| Authors(kana) | |
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| Organization | *Department of Nuclear Medicine, ***Department of Internal Medicine, ****Clinical Laboratory Medicine, Gunma University School of Medicine, **Tano General Hospital |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 32 |
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| Number | 7 |
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| Page | 695-701 |
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| Year/Month | 1995/7 |
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| Article | Report |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | TRAb Dade kit is a radioreceptor assay that detects autoantibodies in serum of patients with thyroid diseases which interfere with the binding of 125I labeled TSH to its receptor. In this paper we examined the basic and clinical usefulness of newly developed TRAb assay kits. The intra and inter assay coefficients of variation were 7.9% and 8.4%, respectively. There was no significant effect of temperature and incubation time on TRAb values. The influence of conjugated, unconjugated bilirubin, hemoglobin and turbidity was not so significant on TRAb values. TRAb values in serum of 68 untreated Graves' disease were above the normal range, but most (78%) treated Graves' patients who became euthyroid after receiving antithyroid drug, showed the normal range. In patients with chronic renal failure TRAb values were also within the normal range. A significant correlation (y=1.0x-0.88, r=0.95) was observed between this TRAb kit and TRAb Cosmic values in various thyroid conditions. This new assay for TRAb was simple, rapid and reproducible. The measurement of serum TRAb values was useful for the evaluation of physiological and pathological function and seemed to be helpful in the management of patients with thyroid disease. |
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| Practice | Clinical medicine |
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| Keywords | TSH receptor antibodies, Radioreceptor assay, 125I labeled TSH, Graves' disease. |
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