Japanese
Title樹立培養細胞への201Tlおよび99mTc-MIBIの取り込みおよび放出に関する検討
Subtitle原著
Authors小森剛*, 松井律夫*, 足立至*, 清水雅史*, 末吉公三*, 楢林勇*
Authors(kana)
Organization*大阪医科大学放射線科
Journal核医学
Volume32
Number7
Page651-658
Year/Month1995/7
Article原著
Publisher日本核医学会
Abstract「要旨」201Tl-chlorideおよび99mTc-MIBIを腫瘍細胞の培養液に加えて, これらの放射性医薬品の腫瘍細胞への取り込みを放射能を測定することによって検討した. 腫瘍細胞としてHeLa細胞を用い, 上記放射性医薬品を加えて培養した細胞を経時的に採取し, 放射性物質の細胞への取り込みを検討し, 次に放射性物質を取り込ませた細胞を放射性物質を含まない培養液で培養し, 放射性物質の放出動態を検討した. さらに, アクチノマイシンD(ACD)で処理した細胞を用い, 細胞の増殖能と放射性物質の取り込みおよび放出との関係を検討した. 放射性物質の60分後の取り込みは細胞数と正相関し, 細胞への特異的な取り込みと確認された. 取り込みの程度は201Tlのほうが99mTc-MIBIより高かったが, 放出の動態はほぼ同じであった. ACD処理細胞では201TlはACDの濃度に正相関して, 放出率が増加する傾向にあり, 99mTc-MIBIは細胞への取り込みが増加したが, 放出率は一定の傾向にあった. 201Tlの腫瘍細胞からの放出率はACD処理によって増加したが, このことは201Tlが悪性腫瘍からのwashoutが遅延するという臨床における知見と合致した.
Practice臨床医学：一般
Keywords201Tl, 99mTc-MIBI, Actinomycin D, Cultured cell, Kinetics.
English
TitleIn Vitro Uptake and Release of 201Tl and 99mTc-MIBI in HeLa Cell
SubtitleOriginal Articles
AuthorsTsuyoshi KOMORI, Ritsuo MATSUI, Itaru ADACHI, Tadafumi SHIMIZU, Kozo SUEYOSHI, Isamu NARABAYASHI
Authors(kana)
OrganizationDepartment of Radiology, Osaka Medical College
JournalThe Japanese Journal of nuclear medicine
Volume32
Number7
Page651-658
Year/Month1995/7
ArticleOriginal article
PublisherTHE JAPANESE SOCIETY OF NUCLEAR MEDICINE
Abstract201Tl is useful in the diagnosis of tumor malignancy determined by the grade of washout rate to the normal tissue especially in lung tumors and thyroid tumors. 99mTc-MIBI, a tracer of myocardial blood flow is also a tumor tracer. We determined whether the growth of the tumor cell is related to the uptake and release of the these tracers in cultured cells (HeLa cell). Cultured HeLa cells were incubated for 1 hr with 10 kBq of either tracer for the kinetic study of cellular uptake and additionally incubated for 90 min with cold medium for the kinetic study of cellular release. These cells cultured with various concentrations of actinomycin D (ACD) were used to examine the correlation between cellular growth and kinetics of these tracers. The uptake in the cell reached a plateau at about 60 min and the uptake at 60 min correlated with the number of the cells and was identified for specific accumulation in the cell. The uptake of 201Tl and 99mTc-MIBI at 60 min was 5.17% and 1.32% respectively of the given dose. The release for 90 min showed almost the same tendency for these tracers. The cellular release of 201Tl with the addition of ACD was increased in association with the concentration of ACD. On the other hand, the cellular release of 99mTc-MIBI showed no change by the addition of ACD. In conclusion, 201Tl showed slower washout in high growth cells than in low growth cells, and could act as an indicator of tumor malignancy by assessment of its washout by the tumor.
PracticeClinical medicine
Keywords201Tl, 99mTc-MIBI, Actinomycin D, Cultured cell, Kinetics.

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