| Japanese |
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| Title | 血中CA125測定用キットBALL ELSA CA125-II KITの基礎的検討 |
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| Subtitle | 技術報告 |
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| Authors | 垣崎純子*, 宮崎忠芳**, 前田知穂* |
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| Organization | *京都府立医科大学附属病院放射線科, **宇治病院 |
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| Journal | 核医学 |
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| Volume | 30 |
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| Number | 7 |
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| Page | 807-812 |
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| Year/Month | 1993/7 |
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| Article | 報告 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」モノクローナル抗体M11を固相抗体にOC125を標識抗体に用いた血中CA125測定用キットBALL ELSA CA125-II KITについて基礎的検討を行った. 測定値はインキュベーション時間, インキュベーション温度, 振盪条件による影響を受けるため測定条件を守る必要があった. 再現性, 回収試験, 希釈試験の結果は良好であった. プロゾーン現象に関しては, 5,000U/ml以上の濃度域に見られた. 最小検出感度は7.5U/mlである. 従来のセントコアCA125 RIAキットと本法との測定値の比較では回帰曲線y=1.058x-0.106, r=0.973と良好であるが個別には乖離する例もあった. 「I. はじめに」1980年Bastらによりヒト卵巣漿液性嚢胞腺癌の腹水細胞培養を免疫原としてモノクローナル抗体(OC125)が作製された. |
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| Practice | 臨床医学：一般 |
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| Keywords | CA125, IRMA, Monoclonal antibody. |
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| English |
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| Title | Basic Studies of BALL ELSA CA125-II KIT for Detection of Serum Cancer Antigen 125 |
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| Subtitle | |
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| Authors | Junko KAKIZAKI*, Tadayoshi MIYAZAKI**, Tomoho MAEDA* |
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| Authors(kana) | |
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| Organization | *Department of Radiology, Kyoto Prefectural University of Medicine, **Uji Hospital |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 30 |
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| Number | 7 |
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| Page | 807-812 |
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| Year/Month | 1993/7 |
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| Article | Report |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary]We made basic studies of BALL ELSA CA125-II KIT which is a solid phase two site immunoradiometric assay. The first monoclonal antibody (M11) is coated on the BALL ELSA solid phase, and the second one (OC125), radiolabeled with 125I, is used as a tracer. Since serum CA125 values varied by incubation time, incubation temperature and agitation speed, the conditions of the assay had to be kept strictly. Intra- and interassay reproducibility, recovery test and dilution test were well satisfied. Prozone phenomenon was observed over 5,000 U/ml. The minimum detectable concentration was 7.5 U/ml. Serum CA125 values measured using BALL ELSA CA125-II KIT was correlated with those measured using conventional CENTOCOR CA125 RIA KIT (y=1.058x-0.106r=0.973), although in the serum of a patient with chronic pancreatitis the discrepancy between serum CA125 values measured using these kits was observed. |
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| Practice | Clinical medicine |
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| Keywords | CA125, IRMA, Monoclonal antibody. |
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