| Japanese |
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| Title | 担ヒト前立腺腺腫ヌードマウスにおける125I標識抗ガンマセミノプロテインモノクローナル抗体の体内動態 |
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| Subtitle | 短報 |
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| Authors | 藤野淡人* |
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| Authors(kana) | |
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| Organization | *北里大学医学部泌尿器科学教室 |
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| Journal | 核医学 |
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| Volume | 30 |
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| Number | 7 |
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| Page | 795-798 |
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| Year/Month | 1993/7 |
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| Article | 報告 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」著者が作成したγ-seminoprotein(γ-Sm)に対するモノクローナル抗体(IgG1, K)およびそのF(ab')2フラグメントを125I標識して, 担ヒト前立腺腺腫ヌードマウスにおける体内動態を観察した. 125I-intact IgGおよび125I-F(ab')2投与後96時間の全身オートラジオグラムでは, いずれによっても腫瘍部に良好な集積を認めた. 組織内放射能濃度測定でも腫瘍部は比較的高値を示した. 抗γ-Sm MoAbによる前立腺腫瘍イムノシンチグラフィへの足掛かりとしたい. 「I. はじめに」1966年, 原らは精漿の免疫電気泳動において, γ-globulin域に特異成分を見いだし, γ-seminoprotein(以下γ-Smと略)と命名した. γ-Smは約10%の糖を含む分子量約29,000の糖蛋白で, ヒト前立腺に特異的に見いだされ, その臓器特異性および種属特異性が知られている. 免疫組織化学的には, 正常前立腺, 前立腺過形成, そして前立腺癌組織いずれにおいても, その腺上皮細胞および腺腔内にγ-Smの局在が認められる. |
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| Practice | 臨床医学：一般 |
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| Keywords | Gamma seminoprotein, Radioiodinated monoclonal antibody, Human benign prostatic hyperplasia xenograft. |
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| English |
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| Title | Biodistribution of 125I Labeled Monoclonal Antibody against Gamma Seminoprotein in the Nude Mice Bearing Human Benign Prostatic Hyperplasia Xenografts |
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| Subtitle | |
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| Authors | Awato FUJINO |
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| Authors(kana) | |
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| Organization | Department of Urology, Kitasato University School of Medicine |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 30 |
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| Number | 7 |
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| Page | 795-798 |
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| Year/Month | 1993/7 |
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| Article | Report |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary]The biodistribution of 125I labeled monoclonal antibody against gamma seminoprotein (γ-Sm) in the nude mice bearing human benign prostatic hyperplasia xenograft was evaluated by whole body autoradiography and by counting of radioactivity in organs. The monoclonal antibody (murine Ig G1, K) to γ-Sm which was established in this institute and its F(ab')2 fragment were radioiodinated using Iodogen method. The autoradiograms demonstrated specific uptake of 125I-intact Ig G as well as 125I-F(ab')2 within the prostatic adenoma xenografts 4 days after intravenous administrations. Radioactivity in the xenograft was relatively higher than those in the liver, kidney or lung. These results suggest that radio-labeled monoclonal antibody against γ-Sm might be applicable for radioimmunodetection of prostatic tumors which produce γ-Sm. |
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| Practice | Clinical medicine |
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| Keywords | Gamma seminoprotein, Radioiodinated monoclonal antibody, Human benign prostatic hyperplasia xenograft. |
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