| Japanese |
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| Title | 血清フリーサイロキシン濃度測定の臨床的意義 - 未希釈血清を用いる限外濾過法および平衡透析法による検討 - |
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| Subtitle | 原著 |
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| Authors | 今野則道*, 萩原康司**, 中田匡信*, 田口英雄** |
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| Organization | *北海道社会保険中央病院 内科, **放射線部 |
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| Journal | 核医学 |
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| Volume | 28 |
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| Number | 7 |
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| Page | 719-728 |
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| Year/Month | 1991/7 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」未希釈血清を平衡透析し, 透析液中のT4濃度を高感度RIAで測定するFree thyroxine (FT4) 測定法 (ED / RIA) を検討し, あわせて未希釈血清を限外濾過し, その濾過液のT4を同様にRIAで測定する方法 (UF / RIA) の結果と比較検討した. T4 RIAの最小測定濃度は1.35ng/Lまたは1.1pg/tubeであった. 両法の精度および再現性はいずれもCVで10%以下であった. 正常範囲はED / RIAで13.0 - 35.6ng/L, UF / RIAで7.1 - 22.6ng/Lであった. 正常人, 甲状腺機能異常, 妊婦, 低TBG血症, 非甲状腺疾患 (NTI) を含めた対象全体での両法のFT4濃度の相関は有意であった (r = 0.831, p < 0.001, n = 99). 妊婦のFT4濃度は妊娠初期の一例を除き, 両法において全例正常範囲内であり, また低TBG症のFT4値も正常であった. NTIではUF / RIAによるFT4は全例正常または正常以上であったが, ED / RIAではlow T4 syndromeにおいて一部正常以下の例があった. NTIにおける両法のFT4値の間には有意な相関関係があった (r = 0.416, p < 0.05, n = 24). 以上からED / RIAによるFT4測定法は, NTIの一部を除きUF / RIA法の結果とよく一致し, しかも従来から煩雑とされてきたED法を簡便化した点で, 臨床的有用性が高く, FT4測定の標準的方法として広く用いられ得るものと考えられる. |
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| Practice | 臨床医学：一般 |
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| Keywords | Free thyroxine, Equilibrium dialysis, Ultrafiltration, Radioimmunoassay, Clinical utility. |
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| English |
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| Title | A Comparison of Measurements of Serum Free Thyroxine Concentration by Equilibrium Dialysis and Ultrafiltration Using Undiluted Serum |
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| Authors | Norimichi KONNO, Kohji HAGIWARA, Masanobu NAKATA, Hideo TAGUCHI |
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| Organization | Department of Internal Medicine, Radiology, Hokkaido Central Hospital for Social Health Insurance |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 28 |
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| Number | 7 |
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| Page | 719-728 |
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| Year/Month | 1991/7 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] We have examined the clinical usefulness for free thyroxine (FT4) measurement by equilibrium dilaysis of undiluted serum and by radio-immunoassay of T4 concentration in the dialysate (ED / RIA). The results were compared with those by ultrafiltration of undiluted serum (UF / RIA), which is believed to offer the best possible approach towards estimation of in vitro serum level of FT4. The precision with both methods was excellent. There was a high degree of correlation of FT4 concentration by both methods (r = 0.831, p < 0.001, n = 99). FT4 concentration by ED / RIA and UF / RIA methods agreed well for hyperthyroidism, hypothroidism, normal pregnancy, and for low T4-binding globulin concentration. In nonthyroidal illness (NTI), FT4 levels were all within or above normal for UF / ED method, while some FT4 values in low T4 syndrome were below normal for ED / RIA method. The effect of progressive serum dilution on FT4 concentration was also examined for both methods. There was no fall in FT4 concentration in sera from normal, hyperthyroid patient and in serum with low T4 syndrome for both methods, but in a serum from low T3 syndrome, there was a fall in FT4 concentration by dilution with UF / RIA method, indicating that serum dilution with ED / RIA method might underestimate FT4 concentration in some NTI sera. From these results it is concluded that the present ED / RIA method is adaptable to clinical laboratory use as a reference standard method for FT4 measurement. |
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| Practice | Clinical medicine |
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| Keywords | Free thyroxine, Equilibrium dialysis, Ultrafiltration, Radioimmunoassay, Clinical utility. |
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