| Japanese |
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| Title | ガンマカメラを利用したDNA-RNAプローブ判定の基礎的検討 |
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| Subtitle | ≪原著≫ |
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| Authors | 五十嵐均*, 久保塚政彦*, 白石明久*, 今井俊幸*, 羽鳥昇*, 井上登美夫**, 冨吉勝美**, 織内昇**, 館野円**, 小出博子**, 平野邦弘***, 佐々木康人**** |
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| Authors(kana) | |
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| Organization | *群馬大学医学部附属病院中央放射線部, **核医学教室, ***群馬県立福祉大学校放射線学科, ****東京大学医学部放射線医学教室 |
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| Journal | 核医学 |
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| Volume | 28 |
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| Number | 2 |
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| Page | 135-141 |
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| Year/Month | 1991/2 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」DNA-RNAプローブ判定の測定手技について, キット指定のオートラジオグラフィによる方法と, ガンマカメラによる方法について比較検討した. ガンマカメラによる測定の最適条件は, 1)コリメータを外し, 測定試料をAlの防護面にできるだけ密着する 2)収集イメージのマトリックサイズは128×128とし, エネルギーウインドは280〜512keVとする 3)収集時間は60分とする の3点であった. また厚さ10mmの銅板でコリメートして得られた空間分解能は, FWHM=6.6mm, FWTM=14.1mmであり, 制動放射の寄与率は8%であった. 従来のオートラジオグラフィによる方法は露光時間が長く, 露光中の温度を-70℃に維持し, 手現像を行うなど, 操作が繁雑である. 一方ガンマカメラによる方法は, 操作上の繁雑さはなく, しかも迅速に測定結果が得られるため, 多量検体処理のスクリーニングに応用できると思われる. |
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| Practice | 臨床医学：一般 |
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| Keywords | DNA-RNA hybridization, DNA-RNA probe assay, Gamma Camera, P-32. |
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| English |
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| Title | Fundamental Studies for the Detection of P-32 in DNA-RNA probe using Gamma Camera |
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| Subtitle | - Original Articles - |
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| Authors | Hitoshi IGARASHI*, Masahiko KUBOZUKA*, Akihisa SHIRAISHI*, Toshiyuki IMAI*, Noboru HATORI*, Tomio INOUE**, Katsumi TOMIYOSHI**, Noboru ORIUCHI**, Madoka TATENO**, Hiroko KOIDE**, Kunihiro HIRANO***, Yasuhito SASAKI**** |
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| Authors(kana) | |
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| Organization | *Department of Central Diagnostic Radiology, Gunma University Hospital, **Department of Nuclear Medicine, Gunma University School of Medicine, ***Department of Radiology, Gunma Prefectural College of Medical Care and Technology, ****Department of Radiology, Tokyo University School of Medicine |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 28 |
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| Number | 2 |
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| Page | 135-141 |
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| Year/Month | 1991/2 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] Comparisons were made between the autoradiography method which follows the fixed order by the manual and the gamma camera method which is modified in this institute. The best conditions for the gamma camera method are as follows. 1) Samples are placed in contact with aluminum protector of the surface of the gamma camera without collimator and measured. 2) Data are collected in 128×128 matrix size and the energy window ranging from 280 to 512 keV. 3) Data collection time needs more than 60 minutes. Under these conditions, spatial resolutions are 6.6 mm of the full width at half maximum (FWHM) and 14.1 mm of the full width at tenth maximum (FWTM). The ratio of Bremsstrahlung to total radiation is 8%. The autoradiography method currently applied in other institutes needs complicated procedures such as exposing for a long time and maintaining the temperature under -70℃ and gives less quantitative data. On the other hand, the gamma camera method needs no such procedure and gives rapid quantitative results. Therefore, it is considered that the gamma camera method is more practical in order to evaluate the DNA-RNA probe than the autoradiography method. |
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| Practice | Clinical medicine |
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| Keywords | DNA-RNA hybridization, DNA-RNA probe assay, Gamma Camera, P-32. |
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