| Japanese |
|---|
| Title | モノクローナル抗体を用いた高感度IgEイムノラジオメトリックアッセイの開発 |
|---|
| Subtitle | 《原著》 |
|---|
| Authors | 坂本春喜*, 今井淳子*, 飯沼一茂*, 早川進*, 柳川佳信*, 池田勲*, 倉田邦夫* |
|---|
| Authors(kana) | |
|---|
| Organization | *ダイナボット株式会社 |
|---|
| Journal | 核医学 |
|---|
| Volume | 27 |
|---|
| Number | 8 |
|---|
| Page | 815-820 |
|---|
| Year/Month | 1990/8 |
|---|
| Article | 原著 |
|---|
| Publisher | 日本核医学会 |
|---|
| Abstract | 「要旨」われわれは, mouse monoclonal antibody(McAb)を用い, 高感度で, 従来にない広い測定範囲をもつヒト血中IgE測定用immunoradiometricassay(IRMA)を開発した. 本IgE IRMAは, IgEのFc部を認識するMcAbをポリスチレンビーズ固相化抗体に, また125I標識抗IgE McAbには, Fab部を認識するMcAbを使用した, いわゆるTwo-site-sandwich methodによるIRMAである. この測定系は, ヒト血清IgM, IgG, アルブミンとの交差反応率がそれぞれ, 6.2×10-6, 1.9×10-6, 6.2×10-7%と特異性が極めて高く, かつ測定感度が6×10-4 IU/tube, 測定範囲が0.01〜20 IU/tubeと高感度で, 広測定範囲を持つことが特徴である. 測定再現性も変動係数(CV%)で2.2〜4.3と良く, Phadebas IgE PRIST, Pharmacia IgE RIAとの比較では, y=1.12x-0.19(r=0.96), およびy=0.97x-5.9(r=0.97)とそれぞれ良好な相関を示すことを認めた. |
|---|
| Practice | 臨床医学：一般 |
|---|
| Keywords | monoclonal antibody, IgE, immunoradiometric assay. |
|---|
| English |
|---|
| Title | The Development of High Sensitive IgE Immunoradiometricassay Using Monoclonal Antibody |
|---|
| Subtitle | - Original Articles - |
|---|
| Authors | Haruki SAKAMOTO, Junko IMAI, Kazushige IINUMA, Susumu HAYAKAWA, Yoshinobu YANAGAWA, Isao IKEDA, Kunio KURATA |
|---|
| Authors(kana) | |
|---|
| Organization | Dainabot Co., Ltd. (Research and Development Depertment) |
|---|
| Journal | The Japanese Journal of nuclear medicine |
|---|
| Volume | 27 |
|---|
| Number | 8 |
|---|
| Page | 815-820 |
|---|
| Year/Month | 1990/8 |
|---|
| Article | Original article |
|---|
| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
|---|
| Abstract | We have developed two-site immunoradiometric assay (IRMA) for quantitation of human serum IgE, using two different anti-IgE monoclonal antibodies which recognize different epitopes on IgE molecule. We obtained 25 different monoclonal antibodies classified seven groups, and then two different monoclonal antibodies were selected for bead coater and tracer after checking the titer and specificity of each antibody. IgE IRMA we developed here showed good performance in terms of specificity, sensitivity and reproducibility. No cross-reactivity to IgG, IgM and albumin in the level of normal range was observed. The sensitivity for IgE assay was 0.6×10-3 IU/tube and measurable range was 0.01-20.0 IU/tube (0.5-103 IU/ml). Coefficient of variations of Intra assay were 2.2-4.3% and average recovery yield was 92.5-108%. Good correlations with Phadebas IgE PRIST and Pharmacia IgE RIA were obtained i.e., y=1.12x-0.19 (r=0.96) and y=0.97x-5.9 (r=-9.7), respectively. These results indicate that the assay system will contribute much to the routine diagnosis for atopic allergy desease and parasitic infections. |
|---|
| Practice | Clinical medicine |
|---|
| Keywords | monoclonal antibody, IgE, immunoradiometric assay. |
|---|