| Japanese |
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| Title | N-isopropyl-P- [I-123] Iodoamphetamineの血液分画への分布 - in vitroでの検討 - |
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| Subtitle | 原著 |
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| Authors | 熊崎智司*, 織内昇**, 冨吉勝美**, 井上登美夫**, 佐々木康人** |
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| Organization | *関東逓信病院呼吸器科, **群馬大学医学部核医学講座 |
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| Journal | 核医学 |
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| Volume | 27 |
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| Number | 3 |
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| Page | 243-248 |
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| Year/Month | 1990/3 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」 I-123IMPの体内挙動解明の一環としてヒト末梢血と混和したI-123IMPの血液成分 (血球・血小板・血漿) への放射能分布をin vitroで検討した. 37℃でincubateし血球・血漿・富血小板血漿成分に分離し, ウェルカウンターで各成分の放射能を測定した. また血球とI-123IMPの結合を評価するための対照として, Tc-99mDTPAと血液を混和後, 生理食塩水で洗浄を繰り返して放射能を測定し, I-123IMPと比較した. 32.0±6.3% (m±1 SD) は血漿に, 68.O±6.3% (m±1 SD) は血球成分に分布した. 富血小板血漿には1.7±1.1% (m±1 SD) が分布したにすぎなかった. 血液分画への分布比率は, incubation時間にかかわらず一定であった. 血球の洗浄結果は, Tc-99mDTPAでは, 血球成分に平均22.5%が分布し, 洗浄ごとにその32±2.1%が上清液中に移行し, 3回洗浄後は平均6.8%が血球に残存したのみであったのに対し, I-123IMPでは, 洗浄上清液に, 血球に対してほぼ一定の比率 (8.20±0.57%) の放射能が認められ, 8回の洗浄後も31.1%が血球成分に残った. I-123IMPの血球成分への分布には, 単に非特異的な結合によるのみならず, 特異的な血球細胞膜への結合機序が関与していると推定される. |
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| Practice | 臨床医学：一般 |
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| Keywords | N-isopropyl-p [I-123] iodoamphetamine (I-123IMP) , Peripheral blood components, In vitro study |
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| English |
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| Title | Distribution of N-Isopropyl-p- [I-123] Iodoamphetamine among the Peripheral Blood Components : An In Vitro Study |
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| Subtitle | Original Articles |
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| Authors | Satoshi KUMAZAKI*, Noboru ORIUCHI**, Katsumi TOMIYOSHI**, Tomio INOUE**, Yasuhito SASAKI** |
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| Authors(kana) | |
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| Organization | *Department of Respiratory Medicine, Kanto Teishin Hospital, **Department of Nuclear Medicine, Gunma University |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 27 |
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| Number | 3 |
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| Page | 243-248 |
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| Year/Month | 1990/3 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] With the purpose to clarify dynamics of Nisopropyl-p- [I-123] iodoamphetamine (I-123IMP) in the blood stream its binding to the peripheral blood components was determined by in vitro experiment. I-123IMP was added to the peripheral venous blood obtained from healthy volunteers to be incubated for different length of time (0-30min) at 37℃. The blood was then separated into blood cells and plasma. From the latter platelet rich plasma were separated. Radioactivity in each blood component was counted in a well type scintillation counter respectively. To evaluate the affinity of I-123IMP to red blood cell the component containing blood cells were washed repeatedly with salines. It was found that the fraction of radioactivity in the blood cell component was 68.0+-6.3% (m+-1 S.D.), which was higher than that in the plasma (32.0%+-6.3%) . The radioactivity in the platelet-rich plasma was only 1.7+-1.1% of the total I-123IMP activity. This percentage did not change by the incubation time. When Tc-99mDTPA was incubated with blood, radioactivity in the blood cell component was only 22.5%, which is further lowered by 32+-2.1% after each washing to reach 6.8% after three times washing. In contrast the radioactivity of I-123IMP in blood cell component remained as high as 31.1% after eight times washing. Almost constant fraction (8.20+-0.57%) of radioactivity was freed into supernate by each washing. These findings suggest that a certain specific binding mechanism is involved in the binding of I-123IMP to red blood cells. |
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| Practice | Clinical medicine |
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| Keywords | N-isopropyl-p [I-123] iodoamphetamine (I-123IMP) , Peripheral blood components, In vitro study |
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