| Japanese |
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| Title | Immunoradiometric assayによるヒト副甲状腺ホルモン -(1-84) の迅速測定法の確立および術中測定法としての応用 |
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| Subtitle | 原著 |
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| Authors | 高坂唯子*, 滋野長平*, 北村暢康*, 岩元則幸***, 塩見一樹*, 李啓充*, 曽根照喜*, 菊池晴揮*, 大田秀一**, 山本逸雄****, 土光茂治*****, 小西淳二* |
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| Authors(kana) | |
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| Organization | *京都大学医学部放射線核医学科, **整形外科, ***京都第一赤十宇病院泌尿器科, ****京都市立病院内科, *****放射線科 |
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| Journal | 核医学 |
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| Volume | 27 |
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| Number | 2 |
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| Page | 117-124 |
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| Year/Month | 1990/2 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」 2-site immunoradiometric assay法に基づくインタクトPTH測定法を改良し血中インタクトPTH濃度を迅速, 正確に測定する方法を開発した. 反応条件を37℃振盪水浴下, 反応時間15分とすることにより, 良好な再現性を得ることができた. 迅速測定法の最小検出感度は27.8pg/mlであり, 広範なホルモン濃度で通常測定法による測定値と良好な正相関を示した (r2=0.979, P<0.001) . 原発性副甲状腺機能亢進症 (単発腺腫, 2例) において, 腺腫への血流遮断後インタクトPTH濃度は急速に低下し, 血流遮断後20分でのインタクトPTH濃度は, 手術前のホルモン濃度のそれぞれ10.6%, 13.5%まで減少した. インタクトPTHの迅速測定法を術中アッセイ法として用いることにより, 副甲状腺機能亢進症の外科的治療の成否を手術中に判断するうえで有益な情報が得られることが期待される. |
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| Practice | 臨床医学：一般 |
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| Keywords | IRMA, Intact PTH, Intraoperative measurement, Rapid assay, Hyperparathyroidism |
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| English |
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| Title | Rapid Measurement of Human Parathyroid Hormone -(1-84) by Immunoradiometric Assay for Use in Intraoperative Determination of Hyperfunctioning Parathyroid Glands |
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| Subtitle | Original Articles |
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| Authors | Tadako KOUSAKA*, Chohei SHIGENO*, Nobuyasu KITAMURA*, Noriyuki IWAMOTO*****, Kazuki SHIOMI*, Kaechoong LEE*, Teruki SONE*, Haruki KIKUCHI*, Shuichi OHTA**, Itsuo YAMAMOTO***, Shigeharu DOKOH****, Junji KONISHI* |
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| Authors(kana) | |
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| Organization | *Department of Radiology and Nuclear Medicine, **Department of Orthopedics, Kyoto University School of Medicine, ***Department of Internal Medicine, ****Department of Radiology, Kyoto City Hospital, *****Department of Urology, Kyoto First Red Cross hospital |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 27 |
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| Number | 2 |
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| Page | 117-124 |
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| Year/Month | 1990/2 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] Rapid measurement of serum intact parathyroid hormone concentration was achieved by modification of an immunoradiometric assay for the hormone. Incubation of serum samples for 15 min at 37℃ under shaking gave optimal results in terms of assay variance and reproducibility : intra-assay CVs were less than 10% over the hormone concentrations of 11-1,600pg/ml ; intra- and inter-assay CVs for two control sera at different hormone levels were less than 12%. The minimal detectable hormone concentration was found at 27.8pg/ml. The serum hormone levels of 43 subjects (31 healthy subjects, 9 patients with primary hyperparathyroidism, and 3 patients with secondary hyperparathyroidism) determined by either rapid or regular assay well correlated with each other (r2=0.979, p<0.001) . In two patients with parathyroid adenoma serum intact PTH levels fell rapidly to 12.1% of the preoperative values 20 min after ligation of the vascular pedicle to the hyperfunctioning glands. We conclude that the modified assay protocol allows rapid, accurate, and simple estimation of intact PTH concentrations, and can be used as an intraoperative measure to aid both diagnosis and surgical cure of hyperparathyroidism. |
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| Practice | Clinical medicine |
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| Keywords | IRMA, Intact PTH, Intraoperative measurement, Rapid assay, Hyperparathyroidism |
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