Japanese
Title糖化低比重リポ蛋白 (Glycosylated LDL) の代謝動態に関する基礎的研究
Subtitle原著
Authors栢森亮*
Authors(kana)
Organization*新潟大学医療技術短期大学部診療放射線技術学科
Journal核医学
Volume26
Number12
Page1513-1521
Year/Month1989/12
Article原著
Publisher日本核医学会
Abstract「要旨」低比重リポ蛋白 (Low Density Lipoprotein: LDL) は動脈硬化の促進因子の一つに挙げられ, 糖尿病では血中の糖化LDLが増加することが指摘されている. 著者は糖化LDLの代謝を明らかにする目的で, 糖化LDLの細胞に対する結合能および家兎の血中からの消失曲線について検討した. 糖化LDLは, 健常人の血漿から超遠心法で得たLDLにD-グルコースを試験管内で非酵素的に反応させて作製した. LDLと糖の結合は, 混和直後から直線的に増加し, 12日後に飽和に達した. 培養細胞に対する糖化LDLの結合能は, native LDLに比べて低値を示した. また, 家兎での血中消失速度 (T 1/2) は, 糖化LDLが85時間であるのに対しnative LDLは10時間であり, 糖化LDLの血中消失が遅延することが判明した. ヒト血中の糖化LDLは, m-aminophenylボロン酸アフィニティー・カラム法により測定した. 糖尿病患者 (21名) の糖化LDL値は10.6±3.0%, 健常者 (18名) では5.25±1.1%であった. 対象者 (39名) における血中の糖化LDL値と血糖値との間には, 有意の相関 (r=0.87, p<0.001) が認められた. また, 血中の糖化LDL値とグリコ・ヘモグロビンAI値との間にも, 正の相関 (r=0.68) が認められた. 以上の成績から, 血中の糖化LDL値は血糖の上昇により増加することが結論された.
Practice臨床医学：一般
KeywordsGlycosylated LDL, Metabolism, Boronic acid affinity chromatography.
English
TitleA Fundamental Study on the Metabolism of Glycosylated Low-Density Lipoprotein
Subtitle
AuthorsRyo KAYAMORI
Authors(kana)
OrganizationDepartment of Radiology, College of Biomedical Technology, Niigata University
JournalThe Japanese Journal of nuclear medicine
Volume26
Number12
Page1513-1521
Year/Month1989/12
ArticleOriginal article
PublisherTHE JAPANESE SOCIETY OF NUCLEAR MEDICINE
Abstract[Summary] Low-density lipoprotein (LDL) is considered to be one of the accelerating factors in atherosclerosis. Recently, it has been pointed out that nonenzymaticaly glycosylated LDL (Glc・LDL) is increased in the blood of diabetic patients. In this study, the binding ability of Glc・LDL to culturer cells and its clearance rate in New Zealand White rabbits were examined to the purpose of elucidating those metabolism. Human LDL was taken from healthy subjects and prepared by the ultracentrifugation method, then was glycosylated in vitro by adding [D]glucose. The incorporation of covalently bound glucose increased linearly from beginning and get to the plateau after 12 days of incubation. Surface binding of 125I-Glc・LDL to cultured human fibroblasts was low degree compared to that of native 131I-LDL. The clearance rate of 125I-Glc・LDL in rabbits was slower than that of native 131I-LDL. The half-time for Glc・LDL was 85 hours, and for native LDL the time was 10 hours. Finally, Glc・LDL in human plasma was measured by a m-aminophenylboronic acid affinity column. Preliminary data revealed that the Glc・LDL level was 10.6+-3.0% in diabetic patients (n=21) and 5.25+-1.1% in non-diabetes (n=18) . The relationships between Glc・LDL and glucose in human plasma for all patients (n=39) showed linearity and the correlation coefficient was about 0.87 (p<0.001) .
PracticeClinical medicine
KeywordsGlycosylated LDL, Metabolism, Boronic acid affinity chromatography.

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