| Japanese |
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| Title | 大腸癌患者血清および組織中thymidine kinase活性の臨床的意義 |
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| Subtitle | 原著 |
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| Authors | 坂本忍*, 嘉和知靖之**, 塚田邦夫**, 三島好雄**, 岡本良平* |
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| Organization | *東京医科歯科大学難治疾患研究所, **第二外科 |
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| Journal | 核医学 |
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| Volume | 25 |
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| Number | 10 |
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| Page | 1089-1094 |
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| Year/Month | 1988/10 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」DNA合成系酵素thymidine kinase(TK)は, 増殖の盛んな組織でその活性が増加していることが知られている. 3H-thymidineよりも125I-iodo deoxyuridineを基質とした測定系が高感度であると報告されており, ヒト大腸癌患者血清TK活性を後者の測定系で測定し, その臨床的意義を検討した. (1)血清TK活性正常値は, 平均2.93±0.85U/lであり, 低TK活性領域では後者の測定系で安定した結果が得られた. (2)大腸癌組織中総TK活性と特異なTKアイソザイム活性との間には正の相関が成立したが, 血清TK活性との間には相関が認められなかった. (3)大腸癌患者で, stage Vおよび再発症例, ことに遠隔転移を伴う症例で, 血清TK活性が高値を示した. 以上の結果, 固形癌症例における血清TK活性測定は, 病態, 病期を把握するうえで有用と思われる. |
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| Practice | 臨床医学：一般 |
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| Keywords | Thymidine kinase(TK), 125I-iodo deoxyuridine(125I-IdUrd), Radicenzyme assay(REA)-kit, Serum TK activity, Human colorectal cancer. |
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| English |
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| Title | Clinical Value of Thymidine Kinase Activities in Sera and Tumor Tissues of Patients with Colorectal Cancer |
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| Subtitle | |
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| Authors | Shinobu SAKAMOTO*, Yasuyuki KAWACHI**, Kunio TSUKADA**, Yoshio MISHIMA**, Ryohei OKAMOTO* |
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| Authors(kana) | |
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| Organization | *Medical Research Institute, **The Second Department of Surgery, Tokyo Medical and Dental University |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 25 |
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| Number | 10 |
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| Page | 1089-1094 |
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| Year/Month | 1988/10 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | DNA-synthesizing enzyme, thymidine kinase (TK) has been known to be high in activity in proliferating tissues. It was reported that radio-enzyme assay (REA) system using 125I-iodo deoxy-uridine as a substrate was more sensitive than that using 3H-thymidine. In the present work, serum TK activity in colorectal cancer bearing patient was measured by the REA system with 125I-iodo deoxyuridine, and its clinical value was investigated. 1) Average activity of serum TK in control group was 2.93+-0.85 U/l. The REA system with 125I-iodo deoxyuridine could detect low activity of serum TK better than that with 3H-thymidine. 2) The activities of total TK and the specific TK-isozyme separated by DEAE-cellulose column chromatography were well correlated positively, but there were no correlation between those and the serum TK activity. 3) In the patients in clinical stage V and recurrence, especially in the cases with distant metastasis, high activities in serum TK were observed. These results indicate that the measurement of serum TK activity may be valuable to determine the clinical stages in the patients with solid tumors. |
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| Practice | Clinical medicine |
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| Keywords | Thymidine kinase(TK), 125I-iodo deoxyuridine(125I-IdUrd), Radicenzyme assay(REA)-kit, Serum TK activity, Human colorectal cancer. |
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