| Japanese |
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| Title | ラット脳における [3H]-QNBの定量的受容体オートラジオグラフィ - ラジオ・レセプター・アッセイとの比較 - |
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| Subtitle | 原著 |
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| Authors | 森厚文*, 柴和弘*, 松田博史**, 辻志郎**, 久田欣一** |
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| Organization | *金沢大学アイソトープ総合センター, **医学部核医学教室 |
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| Journal | 核医学 |
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| Volume | 25 |
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| Number | 6 |
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| Page | 533-541 |
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| Year/Month | 1988/6 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」 受容体オートラジオグラフィとラジオ・レセプター・アッセイ (RRA) より算出した定量値に相違があるといわれている [3H]-quinuclidinyl benzilate (QNB) を用いて, 両法の比較を中心に基礎的検討を試みた. 受容体オートラジオグラフィを文献と同一条件 (室温, インキュベーション時間2時間) で行うと, Scatchard解析は困難であったが, インキュベーション時間を長くすると結合量が増加し解析が可能となった. インキュベーション時間を6〜8時間にしてScatchard解析すると, RRAと近似の定量値 (最大結合量およびみかけの解離定数値) が得られ, 速度論モデルを用いて算出した解離定数値も, RRAとほぼ同じ値が得られた. [H3]-QNBは異性化 (isomerization) することが知られているが, モデル式に忠実な解析法を考案し, 従来より報告されている解析法と比較した. その結果われわれの解析法の方が実験データとよく一致した値を得ることができ, その妥当性が示唆された. |
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| Practice | 臨床医学：一般 |
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| Keywords | Receptor autoradiography, Radioreceptor assay, [3H]-quinuclidinyl benzilate, Muscarinic acetylcholine, Brain |
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| English |
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| Title | Quantitative Receptor Autoradiography using [3H]-Quinuclidinyl Benzilate Binding in the Rat Brain - A Comparison with Radio-receptor Assay - |
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| Subtitle | Original Articles |
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| Authors | Hirofumi MORI*, Kazuhiro SHIBA*, Hiroshi MATSUDA**, Shiro TSUJI**, Kinichi HlSADA** |
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| Authors(kana) | |
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| Organization | *Radioisotope Center, **Department of Nuclear Medicine, Kanazawa University |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 25 |
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| Number | 6 |
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| Page | 533-541 |
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| Year/Month | 1988/6 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] Receptor autoradiography has several important advantages over radio-receptor assay (RRA) which is still widely utilized. However, it has been said that there are significant differences in the binding characteristics of muscarinic receptors observed in tissue slices versus homogenates. We compared receptor autoradiography with RRA, in terms of the binding kinetics for [3H]-quinuclidinyl benzilate (QNB) in the rat striatum. Receptor autoradiography according to experimental condition published in literature showed a distortion of the saturation curve at low concentration of [3H]-QNB and Scatchard analysis was difficult. However, the binding kinetics obtained with tissue slices depended on the incubation time and gradually approached the values with tissue homogenates closer with the incubation time. On the optimal condition, the results by both methods agreed well. It is well known that the association kinetics of the binding of [3H]-QNB follows the two-step isomerization process. Therefore, our new mathematical analysis of two-step isomerization model was compared with the previously reported analysis. The values for dissociation constant (Kd) from non-isomerization model, apparent Kd from our new analysis, and apparent Kd from the previously reported analysis were 11.7pM, 10.8pM, and 3.5pM, respectively. Accordingly, the trustworthiness of our analysis was clearly demonstrated. This new correct kinetic analysis of two step isomerization model is expected to elucidate the complicated ligand-receptor interaction in various animal models. |
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| Practice | Clinical medicine |
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| Keywords | Receptor autoradiography, Radioreceptor assay, [3H]-quinuclidinyl benzilate, Muscarinic acetylcholine, Brain |
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