Japanese
Title肝細胞膜レセプター結合性放射性薬剤99mTc標識Galactosyl Neoglycoalbuminによる肝機能評価の基礎的検討と臨床応用
Subtitle原著
Authors工藤正俊*, 藤堂彰男*, 池窪勝治**, 日野恵**, 伊藤秀臣**, 山口晴二**, 才木康彦**, 山本和高***, 米倉義晴***, 鳥塚莞爾***, 堀内和子****, 横山陽****, David R. VERA*****, Robert C. STADALNIK*****
Authors(kana)
Organization*神戸市立中央市民病院消化器センター内科, **核医学科, ***京都大学医学部核医学科, ****薬学部放射性薬品化学教室, *****University of California, Davis Medical Center
Journal核医学
Volume24
Number11
Page1653-1662
Year/Month1987/11
Article原著
Publisher日本核医学会
Abstract「要旨」Ashwellらにより見いだされたasialoglycoprotein receptor (ASGPR) は哺乳動物の肝細胞膜表面にのみ存在し, その数は種々の肝臓の病的状態に応じて増減することが知られている. このASGPRを介して代謝されることが証明されている合成糖タンパクGalactosyl Neoglycoalbumin (NGA) を99mTcで標識し, 基礎的および臨床的検討を行った. 標識率は平均95%以上で標識後4時間は全く安定であった. 正常ラットにおける99mTc-NGAの生体内分布では, 肝臓が唯一の標的臓器であり, その動態は従来のコロイド製剤や胆道排泄性製剤とは全く異なり, 肝臓への集積後, 腎臓および胆道系へ排泄されることが証明された. 健常人での99mTc-NGAの動態画像は静注投与後30分において良好な肝イメージが得られ60分より胆道系, 尿路系の描出が見られた. また各種肝疾患における99mTc-NGAの肝臓でのtime activity curveはその機能予備力をよく反映し, 新しい肝機能診断薬として有用であると考えられた.
Practice臨床医学：一般
KeywordsAsialoglycoprotein receptor, Tc-99m-Galactosyl-Neoglycoalbumin, Receptor binding radiolabeled ligand, Hepatic functional reserve.
English
TitleEstimation of Hepatic Functional Reserve by Asialoglycoprotein Receptor-binding, Radiolabeled Synthetic Ligand "Tc-99m-Galactosyl-Neoglycoalbumin" - Preclinical and Clinical Studies -
SubtitleOriginal Articles
AuthorsMasatoshi KUDO*, Akio TODO*, Katsuji IKEKUBO**, Megumu HINO**, Hidetomi ITO**, Haruji YAMAGUCHI**, Yasuhiko SAIKI**, Kazutaka YAMAMOTO***, Yoshiharu YONEKURA***, Kanji TORIZUKA***, Kazuko HORIUCHI****, Akira YOKOYAMA****, David R. VERA*****, Robert C. STADALNIK*****
Authors(kana)
Organization*Department of Gastroenterology, **Department of Nuclear Medicine, Kobe City General Hospital, ***Department of Nuclear Medicine, Kyoto University School of Medicine, ****Division of Radiopharmaceutical Sciences, Kyoto University School of Pharmaceutical Sciences, *****Department of Nuclear Medicine, University of California Davis Medical Center
JournalThe Japanese Journal of nuclear medicine
Volume24
Number11
Page1653-1662
Year/Month1987/11
ArticleOriginal article
PublisherTHE JAPANESE SOCIETY OF NUCLEAR MEDICINE
Abstract[Summary] Tc-99m-Galactosyl-Neoglycoalbumin (Tc-99m-NGA) is a receptor binding radiolabeled ligand, which binds to the asialoglycoprotein receptor of the plasma membrane of hepatocytes. NGA was prepared by covalently coupling D-galactose to normal human albumin. NGA was labeled with technetium-99m according to the electrolytic method by Benjamin, which produced Tc-99m-NGA with labeling yield in excess of 95%. Tc-99m-NGA was proved to be stable for at least 4 hours as determined by high performance liquid chromatography. Biodistribution studies of Tc-99m-NGA in normal rats demonstrated that liver is a sole target organ of Tc-99m-NGA. The lack of hepatic uptake of Tc-99m-NGA in healthy chicken corresponded to the lack of the asialoglycoprotein receptors in avian species. Tc-99m-NGA study in a normal volunteer showed apparently different images from those of Tc-99m-pyridoxyl-5-methyltryptophan (PMT) or Tc-99m phytate, suggesting that Tc-99m-NGA is a novel liver imaging radiopharmaceutical. The estimation of the time activity curves in the liver corresponded to the hepatic functional reserves which were evaluated by existing method in 9 clinical cases (2: Normal volunteers, 1: Chronic Active Hepatitis, 1: Liver Cirrhosis (Child A) , 2: Liver Cirrhosis (Child B) , 3: Liver Cirrhosis (Child C) ) . The NGA is useful as an imaging agent as well as in the estimation of the hepatic functional reserve, as the dynamic curves of the liver correlates to the asialoglycoprotein receptor concentration.
PracticeClinical medicine
KeywordsAsialoglycoprotein receptor, Tc-99m-Galactosyl-Neoglycoalbumin, Receptor binding radiolabeled ligand, Hepatic functional reserve.

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