| Japanese |
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| Title | モノクローナル抗体NCC-ST-439, NCC-ST-433による大腸癌 (CO-4), 胃癌 (H-111) シンチグラフィの基礎的検討 |
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| Subtitle | 原著 |
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| Authors | 中村佳代子*, 塚谷泰司*, 西口郁*, 久保敦司*, 橋本省三*, 大石崇**, 渡辺昌彦**, 小平進**, 阿部令彦**, 広橋説雄***, 下里幸雄*** |
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| Authors(kana) | |
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| Organization | *慶應義塾大学医学部放射線科, **外科, ***国立がんセンター病理部 |
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| Journal | 核医学 |
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| Volume | 24 |
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| Number | 7 |
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| Page | 963-973 |
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| Year/Month | 1987/7 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」ヌードマウスに植えつけたヒトの胃癌(St-4)を免疫原として得られたモノクローナル抗体(MoAb, IgM), NCC-ST-439とNCC-ST-433を125Iにて標識し, おのおのを大腸癌(CO-4), 胃癌(H-111)を植えつけたヌードマウスに投与して腫瘍シンチグラフィ, および, 各抗体の生体内動態を検討した. NCC-ST-439(五量体)を投与後5日目にCO-4の腫瘍像を得ることができた. 抗体を単量体にすると鮮明な腫瘍像が3日目に得られたが, 腫瘍に取り込まれた抗体の量は30%低下していた. また, いずれの抗体を投与しても, 肝, 脾臓への非特異的分布が顕著であった. これに対して, NCC-ST-433は胃癌H-111に速やかに蓄積し, 投与後3日目に非常に明瞭な腫瘍像が得られた. 7日目の腫瘍と血液との取り込み量の比は7.8にも達し, 他の組織への非特異的分布も低かった. また, H-111が大きくなるにつれてNCC-ST-433の取り込み量が増加した. NCC-ST-433は胃癌の腫瘍シンチグラフィに有望なMoAbと言える. |
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| Practice | 臨床医学：一般 |
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| Keywords | Monoclonal antibody, NCC-ST-439, NCC-ST-433, Radioimmunodetection. |
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| English |
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| Title | Radioimmunoimaging of Human Colon and Gastric Cancers Xenografts by NCC-ST-439 and NCC-ST-433 Monoclonal Antibodies |
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| Subtitle | Original Articles |
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| Authors | Kayoko NAKAMURA*, Yasushi TSUKATANI*, Iku NISHIGUCHI*, Atsushi KUBO*, Shozo HASHIMOTO*, Takashi OHISHI**, Masahiko WATANABE**, Susumu KODAIRA**, Osahiko ABE**, Setsuo HIROHASHI***, Yukio SHIMOSATO*** |
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| Authors(kana) | |
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| Organization | *Department of Radiology, School of Medicine, Keio University, **Department of Surgery, School of Medicine, Keio University, ***Pathology Division, National Cancer Center Research Institute |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 24 |
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| Number | 7 |
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| Page | 963-973 |
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| Year/Month | 1987/7 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary]Both NCC-ST-439 and NCC-ST-433 are monoclonal antibodies raised against human gastric cancer (St-4) xenografts in nude mice. Imaging and localization experiments were performed by injecting I-125 labeled antibodies into nude mice bearing CO-4 (colon carcinoma) and H-111 (gastric carcinoma). There was uptake of NCC-ST-439 (polymer) into the CO-4, though it was not clearly visualized until 5 days post injection. By injecting NCC-ST-439 (monomer), CO-4 was better seen at day 3, while average accumulation into the tumors decreased compared with NCC-ST-439 (polymer). High radioactivities were observed in the liver and spleen, which was probably due to the immunocomplex with the antigen in the blood. NCC-ST-433 was selectively accumulated into the H-111 with tumor to blood ratio 7.8 at day 7, without significant uptake into the liver and spleen. Significant correlation was also found between the tumor uptake level of NCC-ST-433 and size of tumors. Excellent images of H-111 were obtained 3 days after the injection. NCC-ST-433 holds promise for the radioimmunodetection of gastric cancers. |
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| Practice | Clinical medicine |
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| Keywords | Monoclonal antibody, NCC-ST-439, NCC-ST-433, Radioimmunodetection. |
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