| Japanese |
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| Title | In-111標識抗大腸癌モノクローナル抗体 (19-9, 17-1A) F(ab')2フラグメントを用いたradioimmunodetectionに関する基礎的検討 (第一報) |
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| Subtitle | 原著 |
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| Authors | 川畑鈴佳*, 小泉潔*, 油野民雄*, 渡辺直人*, 秀毛範至*, 絹谷清剛*, 利波紀久*, 久田欣一*, 小西孝司**, 宮崎逸夫**, 高橋豊*** |
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| Authors(kana) | |
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| Organization | *金沢大学医学部核医学科, **第二外科, ***金沢大学がん研究所付属病院外科 |
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| Journal | 核医学 |
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| Volume | 24 |
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| Number | 7 |
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| Page | 919-925 |
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| Year/Month | 1987/7 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」抗大腸癌モノクローナル抗体である19-9, 17-1AのF(ab')2フラグメントをIn-111で標識し, radioimmunodetectionにおける有用性を, 担癌ヌードマウスによるイメージングおよび手術により得られたヒト癌組織とのインビトロ・アッセイにより検討した. ヒト胃癌を担癌したヌードマウスでのイメージングではIn-111 19-9F(ab')2投与後8時間で腫瘍部は明瞭に描画され, 72時間後での腫瘍への集積率は, 10.8%dose/g, 腫瘍対血液比は58.6であり, Ga-67投与の場合のそれぞれ3.1%dose/g, および8.6と比較して, 優れた値を示した. ヒト癌組織とのインビトロ・アッセイでは, In-111 19-9F(ab')2では, 大腸癌の36%(4/11例), 胃癌の50%(3/6例)で陽性であり, In-111 17-1AF(ab')2では, 大腸癌の73%(8/11例), 胃癌の33%(2/6例)で陽性であった. 以上より, In-111標識19-9F(ab')2, 17-1AF(ab')2は, radioimmunodetectionに有用であると思われた. |
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| Practice | 臨床医学：一般 |
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| Keywords | Monoclonal antibody, Radioimmunodetection, 19-9, 17-1A. |
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| English |
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| Title | Study of Radioimmunodetection with In-111 Labeled Monoclonal Anti-colorectal Carcinoma Antibody (19-9, 17-1A) F(ab')2 Fragments |
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| Subtitle | Original Articles |
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| Authors | Suzuka KAWABATA*, Kiyoshi KOIZUMI*, Tamio ABURANO*, Naoto WATANABE*, Noriyuki SHUKE*, Seigou KINUYA*, Norihisa TONAMI*, Kinichi HISADA*, Kouji KONISHI**, Itsuo MIYAZAKI**, Yutaka TAKAHASHI*** |
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| Authors(kana) | |
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| Organization | *Department of Nuclear Medicine, **Second Department of Surgery, School of Medicine, Kanazawa University, ***Department of Surgery, Cancer Research Institute Hospital, Kanazawa University |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 24 |
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| Number | 7 |
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| Page | 919-925 |
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| Year/Month | 1987/7 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary]F(ab')2 fragments of monoclonal anti-colorectal carcinoma antibodies 19-9 and 17-1A, which react with tumor associated antigens found on human colorectal, gastric and other carcinomas, were labeled with In-111. Scintiphotos of athymic mice xenografted human gastric cancer demonstrated good tumor localization 8 hours after injection of In-111 19-9 F(ab')2. Biodistribution of this fragment at 72 hours after injection showed good tumor uptake of 10.8% ID/g tissue and tumor/blood ratio of 58.6, though Ga-67 showed 3.1%ID/g tissue and 8.6 respectively. The in vitro binding affinities of In-111 labeled 19-9 F(ab')2 and 17-1A F(ab')2 to human cancer tissues were also studied. Surgically removed tumor tissues were minced and incubated with the labeled fragments in a microplate. Specific bindings of those F(ab')2 fragments were obtained by subtraction of nonspecific binding of In-111 labeled control mouse IgG F(ab')2. Specific binding of In-111 19-9 F(ab')2 was apparently observed in 36% (4/11cases) of colorectal cancer tissues and in 50% (3/6 cases) of gastric cancer tissues. Specific binding of In-111 17-1A F(ab')2 was also observed in 73% (8/11 cases) of colorectal cancer tissues and in 33% (2/6 cases) of gastric cancer tissues. These results indicate In-111 19-9 F(ab')2 and 17-1A F(ab')2 may be useful for radioimmunodetection of human colorectal and gastric cancers. |
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| Practice | Clinical medicine |
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| Keywords | Monoclonal antibody, Radioimmunodetection, 19-9, 17-1A. |
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