| Japanese |
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| Title | 2,2-dimethoxypropaneを用いた化学洗浄法による定量的二重標識オートラジオグラフィ - 基礎的検討について - |
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| Subtitle | 研究速報 |
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| Authors | 隅屋寿*, 松田博史*, 辻志郎*, 大場洋*, 寺田一志*, 今井啓子*, 久田欣一*, 池田清延**, 柴和弘***, 森厚文***, 関宏恭**** |
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| Authors(kana) | |
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| Organization | *金沢大学医学部核医学教室, **脳神経外科学教室, ***アイソトープ総合センター, ****富山医科薬科大学放射線科学教室 |
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| Journal | 核医学 |
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| Volume | 24 |
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| Number | 3 |
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| Page | 315-318 |
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| Year/Month | 1987/3 |
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| Article | 報告 |
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| Publisher | 日本核医学会 |
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| Abstract | 「I. はじめに」近年, 同一モデルに二種類のアイソトープを投与して血流と代謝を同時に測定する二重標識オートラジオグラフィ法が数多く報告されている. これには二つの核種の半減期の差を利用する半減期法といずれか一方の核種を洗い出す化学洗浄法がある. N-isopropyl-p-125I-iodoamphetamine(125I-IMP)は脳血流用剤として臨床にも使われている123I-IMPの125I標識物質である. IMPとiodoantipyrineによる脳血流値は一致することが報告されており, またIMPの方が計算が簡便であることはすでに著者らが報告した. 今回著者らは, この125I-IMPと3H-2-deoxyglucose(3H-DG)による二重標識オートラジオグラフィを2,2-dimethoxypropane(DMP)を用いた化学洗浄法により施行するための基礎的検討を行い, また実際のオートラジオグラムで血流と代謝の独立したイメージが得られたので報告する. |
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| Practice | 臨床医学：一般 |
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| Keywords | Double-labeled autoradiography, N-isopropyl-p-125I-iodoamphetamine, 3H-2-deoxy-glucose, Cerebral blood flow, Cerebral glucose metabolism. |
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| English |
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| Title | Quantitative Double-labeled Autoradiography by Chemical Washing Method Using 2,2-dimethoxypropane |
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| Authors | Hisashi SUMIYA*, Hiroshi MATSUDA*, Shiro TSUJI*, Hiroshi OHBA*, Hitoshi TERADA*, Keiko IMAI*, Kinichi HISADA*, Kiyonobu IKEDA**, Kazuhiro SHIBA***, Hirofumi MORI***, Hiroyasu SEKI**** |
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| Authors(kana) | |
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| Organization | *Department of Nuclear Medicine, **Neurosurgery, School of Medicine, ***Radioisotope Center, Kanazawa University, ****Department of Radiology, Toyama Medical and Pharmaceutical University |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 24 |
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| Number | 3 |
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| Page | 315-318 |
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| Year/Month | 1987/3 |
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| Article | Report |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary]We performed a double-labeled autoradiographic technique by chemical washing using 2,2-dimethoxypropane (DMP). N-isopropyl-p-125I-iodoamphetamine (125I-IMP) and 3H-2-deoxyglucose (3H-DG) were used for the measurement of cerebral blood flow (CBF) and cerebral metabolic rate for glucose (CMRgl) respectively. CBF image was obtained by 125I-IMP and X-Omat film (Kodak) with thin polyethylene membrane of 6 μ thickness that shielded the beta-ray of 3H-DG of the slices. CMRgl image was obtained by 3H-DG and ultrofilm (LKB) by washing out 125I-IMP with DMP. DMP removed more than 98% of 125I-IMP of brain slices but did not influence the content and distribution of 3H-DG. No exposure was observed after chemical washing when the thin membrane was put on the slices, which indicates that 125I-IMP was almost completely removed by DMP. This autoradiographic technique seems to be useful for evaluating CBF and CMRgl simultaneously under various conditions, since it has quite small cross-contamination and little restriction of shelf-life of a tracer. |
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| Practice | Clinical medicine |
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| Keywords | Double-labeled autoradiography, N-isopropyl-p-125I-iodoamphetamine, 3H-2-deoxy-glucose, Cerebral blood flow, Cerebral glucose metabolism. |
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