| Japanese |
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| Title | アシアロ糖タンパク - アシアロ糖タンパク受容体系を応用した新しい肝機能検査法の開発 - 第3報 99mTc標識ネオ糖タンパククリアランス曲線解析による肝機能検査法の検討 - |
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| Subtitle | 《原著》 |
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| Authors | 羽間弘*, 河相吉**, 久保田佳嗣*, 小島通真**, 村瀬登志彦*, 奥野裕康*, 内藤雄二*, 西山豊**, 鷲野弘明****, 長谷川武夫**, 沢村隆也*, 田代裕***, 田中敬正**, 塩崎安子*, 鮫島美子* |
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| Organization | *関西医科大学第三内科, **放射線科, ***第一生理, ****日本メジフィジックス社 |
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| Journal | 核医学 |
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| Volume | 23 |
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| Number | 7 |
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| Page | 917-926 |
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| Year/Month | 1986/7 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」: アシアロ糖タンパク - アシアロ糖タンパク受容体系を応用した肝シンチグラフィーによる新しい肝機能検査法の有用性を検討した. アシアロ糖タンパクと生理的に等価に肝細胞に取り込まれる合成ネオ糖タンパクを, 99mTcで標識し, ガラクトサミン急性肝障害およびCCl4慢性肝障害ラットに50μg/100g体重を投与し, 肝シンチグラフィーにより, クリアランス曲線を作製した. ついで, 肝クリアランスならびに受容体活性が, 急性肝障害では血清GOT値, コレステロールエステル比 (E/T比) , 慢性肝障害については肝hydroxy proline (Hyp) 含量, 薬物代謝酵素含量との関係を検索した. また, 急性および慢性肝障害における, 肝クリアランスと受容体活性の関係を比較検討し, 以下の成績を得た. すなわち, 肝障害時には, 99mTcネオ糖タンパククリアランスの延長と受容体活性の低下がみられるが, これらは, 急性肝障害ではGOTの上昇およびE/T比の低下と, 慢性肝障害においては肝Hyp含量の増加およびcytochrome P450, cytochrome b5含量の減少と密に相関した. また, 肝障害時の肝クリアランスは, 受容体活性に規定されて変動することが明らかとなった. 以上により99mTcネオ糖タンパククリアランス曲線解析による肝クリアランスの測定は, 肝障害度を鋭敏に反映する新しい肝機能検査法になると考える. |
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| Practice | 臨床医学：一般 |
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| Keywords | Liver function test, Asialoglycoprotein, Asialoglycoprotein receptor, Neoglycoprotein, Liver scintigraphy. |
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| English |
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| Title | A New Liver Function Test Using the Asialoglycoprotein-receptor System on the Liver Cell Membrane : II. Evaluation for Usefulness by Liver Function Test Using the Tc-99m-Neoglycoprotein |
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| Subtitle | Original Articles |
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| Authors | Hiroshi HAZAMA*, Soukichi KAWA**, Yoshitsugu KUBOTA*, Michimasa KOJIMA**, Toshihiko MURASE*, Hiroyasu OKUNO*, Yuji NAITOH*, Yutaka NISHIYAMA**, Koumei WASHINO****, Takeo HASEGAWA**, Takaya SAWAMURA*, Yutaka TASHIRO***, Yoshimasa TANAKA**, Yasuko SHIOZAKI*, Yoshiko SAMESHIMA* |
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| Authors(kana) | |
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| Organization | *The Third Department of Internal Medicine, **Department of Radiology, ***The First Department of Physiology, Kansai Medical University, ****Nihon Medi-Physics Co., Ltd. |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 23 |
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| Number | 7 |
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| Page | 917-926 |
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| Year/Month | 1986/7 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] : We evaluated the vilidity of a new liver function test using liver scintigraphy based on the asialoglycoprotein (ASGP) receptor system on the liver cell membrane in rats with galactosamine-induced acute liver disorder and those with carbon tetrachloride-induced chronic liver disorder. Neoglycoprotein (GHSA) produced by combining human serum albumin with 32 galactose units was labeled with 99mTc and administered (50μg/100 g body weight) to rats with acute or chronic liver disorder. Clearance curves were produced based on liver scintigrams and analysed using the two-compartment model to obtain parameters. In acute liver disorder, the prolongation of 99mTc-GHSA clearance and the decrease in ASGP receptor activities correlated well to the increase in serum GOT and the decrease in the esterified to total cholesterol ratio (E/T ratio) ; in chronic liver disorder, they correlated significantly to the increase in the content of liver hydroxyproline (Hyp) which increased in proportion to the severity of liver fibrosis studied histologically, and to the decrease in the contents of cytochrome P-450 and cytochrome b5 in liver microsomes. Significant correlation was observed between the prolongation of 99mTc-GHSA clearance and the decrease in ASGP receptor activities in both acute and chronic liver disorders. These findings indicate that the measurement of 99mTc-GHSA clearance can be a new liver function test sensitively reflecting the severity of liver damage. |
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| Practice | Clinical medicine |
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| Keywords | Liver function test, Asialoglycoprotein, Asialoglycoprotein receptor, Neoglycoprotein, Liver scintigraphy. |
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