| Japanese |
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| Title | ガリウム標識抗体を用いる腫瘍のradioimmunodetection : ガリウム標識の抗体活性に及ぼす影響について |
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| Subtitle | 原著 |
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| Authors | 古川高子*, 遠藤啓吾***, 大桃善朗****, 阪原晴海***, 小泉満***, 中島鉄夫***, 福永仁夫*, 森田陸司*, 平井秀松*****, 横山陽**, 鳥塚莞爾*** |
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| Authors(kana) | |
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| Organization | *川崎医科大学核医学科, **京都大学薬学部, ***医学部, ****大阪薬科大学, *****北海道大学医学部 |
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| Journal | 核医学 |
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| Volume | 23 |
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| Number | 4 |
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| Page | 337-343 |
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| Year/Month | 1986/4 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」抗ヒトα-fetoproteinポリクローナル抗体, 抗ヒトthyroglobulinモノクローナル抗体, 抗ヒトchorionic gonadotropinモノクローナル抗体などの腫瘍関連抗原に対する抗体の67Ga標識法について基礎的な検討を行った. deferoxamine(DF)をbifunctional chelating agentとして用いて得られた67Ga標識抗体は, Scatchard plot解析から, 抗原に対する結合親和定数, 結合能ともに変化せず, 抗体活性が保持されていることが示された. また, 67Ga標識抗体が投与されたマウスの血漿に第2抗体を添加した実験より, 67Ga標識抗体はin vivoでも安定であることが確認された. 標識はDF結合抗体に67Gaを混和するだけで可能であり, 簡単で, しかもきわめて短時間に終了する利点がみられた. 抗体活性の完全な保持と安定性とを同時に満足できる本標識方法は, 腫瘍のradioimmunodetectionに有用であると期待される. |
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| Practice | 臨床医学：一般 |
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| Keywords | Radioimmunodetection, Bifunctional chelating agent, 67Ga, Antibody. |
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| English |
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| Title | Radioimmunodetection of Tumor with Ga-67 Labeled Antibodies : Effect of Radiolabeling on the Antibody Activities |
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| Subtitle | Original Articles |
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| Authors | Takako FURUKAWA, Keigo ENDO, Yoshiro OHMOMO, Harumi SAKAHARA, Mitsuru KOIZUMI, Tetsuo NAKASHIMA, Masao FUKUNAGA, Rikushi MORITA, Hidematsu HIRAI, Akira YOKOYAMA, Kanji TORIZUKA |
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| Organization | Department of Nuclear Medicine, Kawasaki Medical School, Osaka Pharmacy College, Kyoto University and Hokkaido University |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 23 |
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| Number | 4 |
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| Page | 337-343 |
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| Year/Month | 1986/4 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | Antibodies against tumor associated antigen ; anti-AFP polyclonal antibody, anti-thyroglobulin monoclonal antibody and anti-hCG monoclonal antibody, were labeled with Ga-67, using deferoxamine (DF) as a bifunctional chelating agent. The immunoreactivity and in vivo stability of the Ga-67 labeled antibodies were examined. The effect of DF conjugation to antibodies on the antigen-binding activity was evaluated by RIA and Scatchard analysis or tanned sheep red blood cell hemagglutination technique. When DF was conjugated to antibody at the molar ratio of 1:1, the antibody activity of the DF-conjugated antibodies was fully retained. Whereas, in heavily conjugated antibodies, the maximum antigen binding capacity was reduced. Biodistribution study in normal mice demonstrated the high in vivo stability of Ga-67 labeled antibodies. The labeling of DF-antibody conjugated with Ga-67 was performed easily and quickly, with a high labeling efficiency, requiring no further purification. Thus, this labeling method, providing in vivo stability of Ga-67 labeled antibody and full retention of immunoreactivity, would be useful for the radioimmunodetection of various cancers. |
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| Practice | Clinical medicine |
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| Keywords | Radioimmunodetection, Bifunctional chelating agent, 67Ga, Antibody. |
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