| Japanese |
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| Title | 放射性ヨード標識Peanut Agglutinin (PNA) の腫瘍親和性の研究 : (第一報) 標識操作によるPNAの生物学的活性の変化の検討 |
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| Subtitle | 原著 |
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| Authors | 横山邦彦*, 渡辺直人*, 川畑鈴佳*, 向加津子*, 小泉潔*, 油野民雄*, 利波紀久*, 久田欣一*, 鷲野弘明**, 越村三郎*** |
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| Authors(kana) | |
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| Organization | *金沢大学医学部核医学科, **日本メジフィジックス(株)技術部, ***金沢大学癌研究所化学療法部 |
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| Journal | 核医学 |
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| Volume | 23 |
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| Number | 1 |
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| Page | 17-24 |
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| Year/Month | 1986/1 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」Thomsen-Friedenreich(T)抗原に強い結合性を有する放射性ヨード標識PNAを用い癌のイメージング診断を行うにあたり, それに先立って今回標識法の基礎的検討を行った. 標識法・標識条件を標識前後のPNAの生物学的活性の変化で評価するため, 分光光度計による簡便かつ鋭敏な半定量的な活性測定法を開発し, それを用いた. クロラミンT法では, 標識後PNAの生物学的活性は50.7%に低下し, in vivoでの体内分布の検討では, 変性による凝集物生成に基づくと考えられる網内系での放射能の停滞がみとめられた. 一方, ヨードゲン法では標識後も生物学的活性の87.9%が保持された. 担癌動物での体内分布の検討でも血中よりの放射能のクリアランスがより早く, 筋肉等の臓器内の放射能の停滞が短いため, より良好な腫瘍対他臓器比が得られた. 以上よりPNAのヨード標識に関しては, クロラミンT法よりヨードゲン法の方が適した方法であると考えられた. |
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| Practice | 臨床医学：一般 |
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| Keywords | Thomsen-Friedenreich (T) antigen, PNA, PNA biological activity, Tumor imaging, Iodogen. |
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| English |
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| Title | Radioiodinated Peanut Agglutinin (PNA) : A Potential New Tumor Seeking Agent |
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| Subtitle | Original Articles |
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| Authors | Kunihiko YOKOYAMA*, Naoto WATANABE*, Suzuka KAWABATA*, Katsuko MUKAI*, Kiyoshi KOIZUMI*, Tamio ABURANO*, Norihisa TONAMI*, Kinichi HISADA*, Komei WASHINO**, Saburo KOSHIMURA*** |
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| Authors(kana) | |
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| Organization | *Department of Nuclear Medicine, School of Medicine, Kanazawa University, **Research and Development Section, Technical Department, Nihon Medi-physics Co., Ltd., ***Department of Experimental Therapeutics, Cancer Research Institutes, Kanazawa University |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 23 |
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| Number | 1 |
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| Page | 17-24 |
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| Year/Month | 1986/1 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | The Thomsen-Friedenreich (T) antigen, β-D-Gal-(1→3)-α-D-GalNAC, is expressed as a reactive form on many human adenocarcinomas, but not on normal or benign tissues. PNA (peanut agglutinin), one of plant lectins, is expected to be a potential tumor seeking agent because of its strong binding affinity for the T antigen. The purpose of this study was to evaluate the difference between chloramine-T method and Iodogen method, and to determine which labeling method was more desirable for radioiodination of PNA. With this point of view we have established the quantitative method to determine the biological activity of PNA. The advantage of this quantitative measurement with a spectrophotometer was simple and sensitive. And we estimated the effect of labeling on the biological activity of PNA by this photometric measurement. In chloramine-T method the biological activity of PNA was decreased to 50.7% and the biodistribution study showed the prolonged retention of the radioactivity in reticuloendothelial system, which was probably due to the formation of macroaggregates. On the other hand, 87.9% of the prelabeled biological activity of PNA was preserved in Iodogen method. Furthermore its blood clearance was more rapid, and the radioactivity in major organs such as liver, spleen, muscle etc. was more rapidly cleared. Rapid organ clearance, combined with a tumor retention allowed to attaine a favorable tumor to background ratio. From these investigations we concluded Iodogen method was more suitable for radioiodination of PNA. |
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| Practice | Clinical medicine |
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| Keywords | Thomsen-Friedenreich (T) antigen, PNA, PNA biological activity, Tumor imaging, Iodogen. |
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