| Japanese |
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| Title | 11CO2のグルコースと尿素への固定 : ポジトロン放出性化合物の迅速分析法によるラット血液を用いた実験研究 |
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| Subtitle | 原著 |
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| Authors | 桜川宣男*,**, 松井晨*, 河野義恭*, 有馬正高**, 里吉栄二郎**, 飯尾正明*** |
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| Organization | *国立武蔵療養所小児神経科, **国立武蔵療養所神経センター, ***国立療養所中野病院 |
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| Journal | 核医学 |
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| Volume | 21 |
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| Number | 11 |
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| Page | 1429-1435 |
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| Year/Month | 1984/11 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」11CO2の体内代謝動態を調べる目的で, 11CO2連続吸入後のラット血液中代謝産物の分析を行った. 血液はトリクロル酢酸で処理し, 新しく開発したdouble column chromatographyで分析した. 各物質の同定には14C-標識化合物を用い, 糖および尿素の定量およびアミノ酸分析を行った. 11CO2 5分間連続吸入後のラット血液中には, 酸不安定成分 (H11CO3-, 11CO2, H2 11CO3) が94.2%, 酸可溶性成分 (中性物質, 解糖系中間代謝産物）が3.9%および酸不溶性成分 (蛋白, 核酸など) が1.9%含まれていた. 酸可溶性成分をdouble column chromatographyで分析した結果, 11C-glucoseは44.9%, 11C-ureaが41.4%を占めていた. なおラジオ液体クロマトおよびDowex (Borate) column chromatographyを施行した結果, 標準品として用いた14C-glucoseと全く同じ溶出パターンを示したことより, 11CO-glucoseの存在が再確認された. 以上より吸気中の11CO2がラット血液中のglucoseとureaに固定されることが証明された. |
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| Practice | 臨床医学：一般 |
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| Keywords | 11CO2, CO2 fixation, Glucose, Urea |
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| English |
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| Title | Incorporation of 11CO2 into Glucose and Urea : Experimental Study by Using Rat Blood which was Analyzed by Newly Developed Methods for Positron Emitting Chemical Compounds |
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| Subtitle | Original Articles |
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| Authors | Norio SAKURAGAWA*,**, Akira MATSUI*, Yoshiyasu KONO*, Masataka ARIMA**, Satoyoshi EIJIRO*, Masaaki IIO*** |
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| Organization | *Department of Pediatric Neurology, National Musashi Institute for Mental Diseases, **National Center for Nervous, Mental and Muscular Diosrderes, ***National Nakano Chest Hospital |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 21 |
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| Number | 11 |
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| Page | 1429-1435 |
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| Year/Month | 1984/11 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] In order to clearify the 11CO2 dynamic metabolism in vivo, we performed the experimental studies by using rats which were exposed to 11CO2 for 5 minutes. Rats were sacrificed soon after 11CO2 exposure. Blood was collected in a heparinized glass contener, homogenized with 0.3 M trichloro acetic acid on ice and separated by centrifugation (4,000×g for 10 min) into the clear supernatant, acid soluble (A-S) fraction, and pellet, acid-insoluble (A-I) fraction. 11C-content of a part of blood was measured by well-type scintilation counter as well as A-S and A-I fraction. An acid-labile (A-L) fraction was estimated by subtraction of A-S and A-I fraction from the 11C-counts of the total blood and appropriate half-life correction was made. A-L fraction (H11CO3-, 11CO2, H211CO3) was 94.2% of the total blood count, A-S fraction (neutral substance, intermediates of glycolysis), 3.9% and A-I fraction (protein, nucleic acid etc), 1.9% respectively. Then A-S fraction was applied to the double column chromatography, which composed of Dowex AG-1 (upper column) and Dowex 50W×8 (lower column). It gave the 5 differents fractions which were identified by using 14C-labelled compounds. Major components in the A-S fraction was 11C-glucose (44.9%) and urea (41.4%). Furthermore, 11C-glucose was identified by radioliquid column chromatography and Dowex (Borate) column chromatography. We interpret these data to suggest that 11CO2 was incorporated into urea in the liver and into glucose in the liver and kidney. Incorporated 11C-glucose was carried out through the body, even into the brain. We proposed the 11CO2 dynamic metabolism in human and made its schematic presentation which probably is useful for 11CO2 dynamic positron emission tomography. |
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| Practice | Clinical medicine |
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| Keywords | 11CO2, CO2 fixation, Glucose, Urea |
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