Japanese
Titleポジトロン放出核種を用いた定量的オートラジオグラフィの試み
Subtitle原著
Authors高橋和弘*, 村上松太郎*, 羽上栄一*, 宍戸文男*, 三浦修一*, 佐々木広*, 菅野巖*, 上村和夫*
Authors(kana)
Organization*秋田県立脳血管研究センター放射線医学研究部
Journal核医学
Volume21
Number11
Page1389-1393
Year/Month1984/11
Article原著
Publisher日本核医学会
Abstract「要旨」ポジトロン放出核種18Fを用いたオートラジオグラフィ (ARG) の定量化を試みた. 18F標識トレーサーは [18F] 2-deoxy-2-fluoro-D-glucose, 動物はラットを用い, 標準線源にはラット肝ホモジネートにさまざまの放射能濃度のトレーサーを均等に混合し, 毎回調製した. なお標準線源はチューブにつめて, 動物組織とともにCMC-Naに包埋し, クライオトームで切片を作り, 動物組織と同一切片内に配列した. オートラジオグラムの黒化度はミクロデンシトメータで測定し, コンピューターに入力し画像化して分析した. 標準線源の黒化度と放射能濃度から標準曲線を求め, それに基づいて各組織の放射能濃度を決めた. 本法で求めた放射能濃度から算出したRIの体内分布は, 組織摘出法で求めた値によく一致した. 本法は11Cや13Nなどのさらに半減期の短い核種を用いたARGの定量化にも利用可能と考えられる.
Practice臨床医学：一般
KeywordsAutoradiography, Rats, 18F-FDG
English
TitleMethod for Quantitative Autoradiography with Positron Emitters
SubtitleOriginal Articles
AuthorsKazuhiro TAKAHASHI, Matsutaro MURAKAMI, Eiichi HAGAMI, Fumio SHISHIDO, Shuichi MIURA, Hiroshi SASAKI, Iwao KANNO, Kazuo UEMURA
Authors(kana)
OrganizationDepartment of Radiology and Nuclear Medicine, Research Institute for Brain and Blood Vessels-AKITA
JournalThe Japanese Journal of nuclear medicine
Volume21
Number11
Page1389-1393
Year/Month1984/11
ArticleOriginal article
PublisherTHE JAPANESE SOCIETY OF NUCLEAR MEDICINE
Abstract[Summary] Quantification of positron autoradiography (ARG) obtained with positron emitter 18F was carried out with a computerized imaging system. Quantitative analysis of ARG is essential in the study of pharmacokinetics of any radiopharmaceuticals in normal and diseased state of animals. Recently, many techniques for quantitative ARG were established using 14C and 3H. But ARG with very short lived positron emitting nuclides (e.g. 11C, 18F, 13N etc.) have not been treated quantitatively because of difficulty to get the calibrated standard sources for quantification of ARG. Now we tried a new technique for quantitative ARG with positron emitter, 18F, in which we made the standard sources for each ARG. A positron emitting radiopharmaceutical [18F] 2-deoxy-2-fluoro-D-glucose (18F-FDG) was administered intravenously to rats. The rats were killed at 60 min after injection, embedded in 5%CMC-Na solution and frozen at -76°. The standard sources which have various concentrations of radioactivity were made of rat liver homogenates containing 18F radioactivity, packed in each tube and put in the CMC-Na surrounding the rat. The frozen CMC-Na block was cut into 20 μm thick sections consisting of rat and standard sources. From these sections ARG was taken by conventional method. The optical densities of ARG of the standard sources were plotted against its net radioactive concentrations measured by the well counter. By means of this relation were can determine the absolute values of radioactive concentration (μCi/ml) at any region of ARG, or which indicates uptake fiction (%dose/ml tissue) for organs.
PracticeClinical medicine
KeywordsAutoradiography, Rats, 18F-FDG

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