| Japanese |
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| Title | 標識抗ヒトα-fetoprotein抗体を用いる腫瘍イメージングの基礎的検討 : 第1編 ヨード標識による抗体活性の変化 |
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| Subtitle | 原著 |
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| Authors | 阪原晴海*, 遠藤啓吾*, 中島鉄夫*, 太田仁八*, 鳥塚莞爾*, 岡田謙一郎**, 吉田修**, 平井秀松*** |
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| Authors(kana) | |
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| Organization | *京都大学医学部核医学科, **泌尿器科, ***北海道大学医学部生化学教室 |
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| Journal | 核医学 |
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| Volume | 21 |
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| Number | 7 |
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| Page | 805-813 |
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| Year/Month | 1984/7 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」ヒトα-fetoprotein(AFP)に対するポリクローナル抗体を用いてヨード標識による抗体活性の変化をみるとともに担癌ヌードマウスにおける131I-標識抗体のAFP産生腫瘍への集積について検討した. 抗体はヒトAFPでウマを免疫して得られた抗血清をaffinity chromatographyで精製したもので, クロラミンT法によりヨード標識した. 標識抗体のAFPへの結合親和定数, 結合能の変化をRIAを用いたScatchard plotで解析したところ, 低濃度のクロラミンTを用い, 抗体1分子に結合するヨード原子数が0.8までの標識では抗体活性はほぼ完全に保たれていることが示された. 131I-標識抗AFP抗体をAFP産生ヒト睾丸腫瘍を移植したヌードマウスに投与して得られたシンチグラムでは腫瘍は陽性描画され, 標識抗体投与後7日目の体内分布も腫瘍/血液比が0.82と対照として用いたAFP非産生ヒト膀胱腫瘍の0.42より有意に高く(P<0.01), 標識抗体のAFP産生腫瘍への特異的な集積を裏付けた. |
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| Practice | 臨床医学：一般 |
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| Keywords | α-fetoprotein, 131I-labeled antibodies, antibody activity, nude mouse, tumor imaging. |
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| English |
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| Title | Basic Studies on the Tumor Imaging Using Antibodies to Human α-Fetoprotein |
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| Subtitle | Original Articles |
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| Authors | Harumi SAKAHARA*, Keigo ENDO*, Tetsuo NAKASHIMA*, Hitoya OHTA*, Kanji TORIZUKA*, Kenichiro OKADA**, Osamu YOSHIDA**, Hidematsu HIRAI*** |
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| Authors(kana) | |
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| Organization | *Department of Nuclear Medicine, **Department of Urology, Kyoto University School of Medicine, ***Department of Biochemistry, Hokkaido University School of Medicine |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 21 |
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| Number | 7 |
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| Page | 805-813 |
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| Year/Month | 1984/7 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary]Using polyclonal antibodies to human α-fetoprotein (AFP), the effect of iodination on the antibody activity and tumor accumulation of radioiodinated antibodies in tumor bearing nude mice were examined. Antibodies, obtained from horse antiserum and purified by affinity chromatography, were iodinated by the chloramine-T method and their antibody activity was evaluated using RIA and Scatchard plot analysis. When high concentrations of chloramine-T were used or more than 2.6 iodine atoms were incorporated per antibody molecule, the antigen binding capacity rather than the affinity constant was affected by the iodination. The antibody activity was completely destroyed at an iodine to antibody molar ratio of 15.4. Antibodies, however, which were iodinated under low concentrations of chloramine-T and contained less than 0.8 iodines per antibody molecule, showed almost full retention of their antibody activity. Nude mice transplanted with AFP producing human testicular tumor or AFP non-producing human urinary bladder tumor were administered intravenously with 131I-labeled antibodies to human AFP. Scintigrams were taken at 1, 2, 4 and 7 days after the injection of labeled antibodies. At day 7, nude mice were sacrificed and organs and tumor were removed, weighed and counted. In nude mice bearing testicular tumor, tumor image became gradually clear with decreasing background activity and tumor to blood ratio, obtained, was 0.82 for testicular tumor compared to 0.42 for bladder tumor. These results indicated a specific in vivo localization of 131I-labeled antihuman AFP antibodies in AFP producing tumor. |
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| Practice | Clinical medicine |
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| Keywords | α-fetoprotein, 131I-labeled antibodies, antibody activity, nude mouse, tumor imaging. |
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