| Japanese |
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| Title | Amerlex Free T3 Kitおよび平衡透析法による血中遊離Triiodothyronine濃度測定法の比較 |
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| Subtitle | 原著 |
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| Authors | 今野則道*, 今寛*, 萩原康司**, 田口英雄** |
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| Organization | *北海道社会保険中央病院内科, **放射線部 |
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| Journal | 核医学 |
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| Volume | 21 |
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| Number | 6 |
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| Page | 687-695 |
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| Year/Month | 1984/6 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」種々の病態における血清遊離T3(FT)3を平衡透析法(ED)およびT3誘導体を用いたAmerlex FT3 RIA kitにより測定し, 両者について比較検討した. 三種類のFT3濃度をもつコントロール血清でのinter-およびintraassay CV(%)は, 両法で9.0%以下であった. 両法について, 血清を稀釈した場合および血清にT3を加えた場合の%FT3をしらべたが, いずれにおいても変化はなく, 本kitでは, 稀釈血清を用いて高FT3値を測定できると考えられた. 125名の正常人から得たFT3の正常範囲はRIAで3.5-9.1 p mol/l, EDで2.1-7.2 p mol/lであった. 甲状腺機能亢進症(n=33), 甲状腺機能低下法(25), 3rd trimester妊婦(17), 甲状腺機能亢進症を合併した妊婦(4), 低TBG血症(4), 27甲状腺疾患(NTI)全体についての両法によるFT3の相関は良好であった(r=0.971, P<0.001). 甲状腺疾患, 低TBG症, 正常T3 NTI群では両法のFT3値の分布は良く一致したが, 妊婦および低T3 NTI群では, ED法によるFT3がほぼ正常であったのに反し, RIA法では正常下限から正常以下に分布した. 以上からRIA法によるFT3測定は, 甲状腺機能異常症のFT3値を知る上できわめて有用であるが, 妊婦, 低T3 NTI群ではED法に比し低値をとり, その評価には慎重を要することが示唆された. |
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| Practice | 臨床医学：一般 |
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| Keywords | Free T3, RIA, Equilibrium dialysis, Diagnostic aids. |
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| English |
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| Title | A Comparison of the Measurements of Serum Free Triiodothyronine Concentration by Amerlex Free T3 RIA Kit and by Equilibrium Dialysis Method |
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| Subtitle | Original Articles |
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| Authors | Norimichi KONNO*, Hiroshi KON*, Kohji HAGIWARA**, Hideo TAGUCHI** |
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| Organization | *Department of Internal Medicine, and **Radiology, Hokkaido Central Hospital for Social Health Insurance |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 21 |
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| Number | 6 |
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| Page | 687-695 |
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| Year/Month | 1984/6 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary]We have assessed a new method of free T3 measurement (Amerlex FT3 RIA kit) and the results obtained were compared with those by equilibrium dialysis method (ED). This method uses a T3-labeled analogue, said to be unreactive with T3-binding proteins in serum, together with an antibody that binds both the analogue and T3. FT3 is assessed by competitive binding of free T3 and the analogue to the antibody binding sites. FT3 was measured in 125 healthy subjects, 33 patients with hyperthyroidism, 25 patients with hypothyroidism, 17 women in the 3rd trimester of pregnancy, 4 pregnant women with hyperthyroidism, 4 subjects with low thyroxine-binding globulin (TBG), and 27 patients with non-thyroidal illnesses (NTI). Inter- and intraassay precisions (CV) for both RIA and ED methods were less than 9% at three different FT3 levels. Serum FT3 fraction was unchanged for both methods when serum was diluted or enriched with different concentration of T3, indicating that FT3 levels can be measured in the diluted sera when FT3 is expected to be higher than the upper limit of RIA standard curve. The normal ranges were 3.5-9.1 p mol/l for RIA, and 2.1-7.2 p mol/l for ED. There was an excellent correlation between FT3 by RIA and ED (r=0.971, n=228, p<0.001). The RIA method gave the expected FT3 levels in hyper- and hypothyroidism, in low TBG, and in NTI with normal serum T3 concentration. However, FT3 by RIA ranged from normal to subnormal in pregnancy and in NTI with low T3 where FT3 by ED were essentially within the normal range. These results indicate that FT3 by Amerlex kit is a rapid and reliable method for quantifying the FT3 levels in hyper- and hypothyroidism, but the FT3 by RIA tends to be lower than that by ED in pregnancy and in NTI with low T3 concentration in serum. |
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| Practice | Clinical medicine |
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| Keywords | Free T3, RIA, Equilibrium dialysis, Diagnostic aids. |
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