| Japanese |
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| Title | 111In-oxineによる白血病細胞標識の基礎的検討と急性白血病における細胞回転の観察への応用 |
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| Subtitle | 原著 |
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| Authors | 高木雄行*, 松田信*, 内田立身*, 刈米重夫* |
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| Organization | *福島県立医科大学第一内科 |
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| Journal | 核医学 |
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| Volume | 21 |
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| Number | 4 |
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| Page | 341-351 |
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| Year/Month | 1984/4 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」111In-oxineによる白血病細胞標識の基礎的検討と, 急性骨髄性白血病 (AML) 5例に対する白血病細胞回転を検索した. 標識率は細胞数が1×108個以上あれば, 室温で20分インキュベートし2回洗浄した後に80.8±3.6%であり, trypanblue排除試験によるviabilityは95.3±2.6%であった. またin vitroで12時間後までの111Inの溶出率は10.0±1.2%であった. AMLにおける標識白血病細胞の血中消失曲線は指数関数的に1相性に減少し, T1/2は9.6〜31.8時間であった. また全血白血病細胞プール (TBLCP) は末梢血白血病細胞数 (LC) と良い正の相関を示した (Y=0.32+1.94X, r=0.99) . 臓器分布の観察では, 標識白血病細胞は肺をすみやかに通過し, 主に脾, 次いで肝, 一部は骨髄に分布した. 脾の放射能は30〜60分で急速に上昇し, 以後はプラトーを呈した. 肝の放射能は10分後から60分後にかけて一時的な減少がみられた. 1例で22時間後に得られた骨髄における白血病細胞の放射能は高値であった. |
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| Practice | 臨床医学：一般 |
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| Keywords | 111In-oxine, Acute leukemia, Leukemic cell kinetics. |
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| English |
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| Title | Fundamental Studies of Leukemic Cell Labeling with 111In-Oxine and Their Applications to Cell Kinetics in Patients with Acute Leukemia |
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| Subtitle | Original Articles |
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| Authors | Yuhkoh TAKAGI, Shin MATSUDA, Tatsumi UCHIDA, Shigeo KARIYONE |
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| Authors(kana) | |
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| Organization | The First Department of Internal Medicine, Fukushima Medical College |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 21 |
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| Number | 4 |
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| Page | 341-351 |
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| Year/Month | 1984/4 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] Fundamental studies of leukemic cell labeling with 111In-oxine and their applications to leukemic cell kinetics in five patients with acute myeloblastic leukemia (AML) were examined. Labeling efficiency of leukemic cells was 80.3+-3.6% for more than 1×108 cells at room temperature for 20 minutes of incubation followed by two times washes. Cell viability determined by means of trypanblue exclusion test was 95.3+-2.6%. In vitro elution rate of 111In from the labeled cells during 12 hours was 10.0+-1.2%. The disappearance curves of labeled leukemic cells in AMLs followed a single exponential fashion, and the half time of disappearance (T 1/2) ranged from 9.6 to 31.8 hours. Total blood leukemic cell pool (TBLCP) calculated with the dilution principles of radioisotopes correlated significantly with the leukemic cell counts (LC) in the peripheral blood (Y=0.32+1.94X, r=0.99) . In the studies of organ distribution which were observed and analized with gamma camera and computer, labeled leukemic cells passed through lungs within 15 minutes. Radioactivity in the spleen increased rapidly for 30-60 minutes, then reached a plateau. Hepatic radioactivity showed a temporary decrease during 10-60 minutes following the moderate accumulation in initial 10 minutes. In two cases, bone marrow was visualized 24 hours after the injection. Radioactivity of the leukemic cells isolated from the bone marrow at 22 hours after the injection in one case was one third of the radioactivity in leukemic cells obtained from the peripheral blood at the same time. |
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| Practice | Clinical medicine |
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| Keywords | 111In-oxine, Acute leukemia, Leukemic cell kinetics. |
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