| Japanese |
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| Title | TSHのradioreceptor assayによるTSH結合阻害性抗体の測定に関する基礎的検討 |
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| Subtitle | 原著 |
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| Authors | 飯田泰啓*, 小西淳二*, 高坂唯子*, 御前隆*, 中島鉄夫*, 遠藤啓吾*, 隈寛二**, 鳥塚莞爾* |
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| Organization | *京都大学医学部放射線核医学科, **隈病院 |
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| Journal | 核医学 |
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| Volume | 20 |
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| Number | 10 |
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| Page | 1415-1423 |
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| Year/Month | 1983/12 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」可溶化ブタ甲状腺膜を用いるTSHのradioreceptor assay (Smith キット) によるTSH-binding inhibitor immunoglobulins (TBII) の測定に関して基礎的検討を行った. Assayの最少検出感度は30μU/mlであった, TBII測定の変動係数は同一assay内で3.2〜4.5%, 異なるassay間で3.7〜25.4%であった. 免疫グロブリン (Ig) 分画を用いるTBIIの正常域は92〜108%で, 未治療バセドウ病26例中22例 (85%) , 特発性粘液水腫6例中2例 (33.3%) で陽性であった. 橋本病, 亜急性甲状腺炎, 腺腫では全例陰性であった. バセドウ病31例のTBII活性はヒト甲状腺膜を用いてのTBII値と有意の正相関 (p<0.001) を示した. 血清を試料として測定したTBII値もIg分画を用いた結果と有意の正相関 (p<0.001) を示した. しかし, 非特異的沈澱が増加するため高γグロブリン血症の場合の測定値の評価に注意を要する. 試料として血漿を用いると125I-TSHの結合率が上昇するためTBII活性を偽陰性と判定する可能性がある. |
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| Practice | 臨床医学：一般 |
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| Keywords | TSH, radioreceptor assay, TSH-binding inhibitor immunoglobulins, Graves' disease |
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| English |
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| Title | Fundamental Studies on Measurement of TSH-binding Inhibitor Immunoglobulins by Using a TSH-radioreceptor Assay |
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| Subtitle | Original Articles |
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| Authors | Yasuhiro IIDA*, Junji KONISHI*, Tadako KOUSAKA*, Takashi MISAKI*, Tetsuo NAKASHIMA*, Keigo ENDO*, Kanji KUMA**, Kanji TORIZUKA* |
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| Authors(kana) | |
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| Organization | *Department of Radiology and Nuclear Medicine, Kyoto University School of Medicine, **Kuma Hospital |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 20 |
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| Number | 10 |
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| Page | 1415-1423 |
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| Year/Month | 1983/12 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] A TSH-radioreceptor assay which uses Lubrolsolubilized porcine thyroid membrane as receptors (B. R. Smith) was evaluated for its usefulness in detecting TSH-binding inhibitor immunoglobulins (TBII) . Sensitivity of the assay was 30μU/ml TSH. Intra-and inter-assay C. V. s in the assay of TBII were 3.2-4.5% and 3.7-25.4%, respectively. When immunoglobulin concentrates were used as samples, relative 125I-TSH binding was 100+-3.6% (mean+-SD) in 21 normal controls, while 22 out of 26 untreated patients with Graves' disease (85%) showed 125I-TSH binding less than 92% of controls. Potent TBII were detected in 3 patients with pretibial myedema and 2 patients with primary myxedema. Results obtained in 31 patients with Graves' disease showed a significant correlation with those obtained by our assay using Tritonsolubilized human receptors (r=0.68, p<0.001) . When serum was used as sample, 20 normals ranged 100+-8.3%. TBII detected in 17 sera correlated well with those obtained by using immunoglobulins (r=0.94, p<0.001) . However, in the case of serum sample, non-specific precipitation of 125I-TSH was elevated with the increasing globulin concentration, requiring an appropriate correction for the non-specific precipetation in the cases with hyperglobulinemia. In the case of plasma sample, TBII activity could be underestimated because of the increase of 125I-TSH precipitation. |
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| Practice | Clinical medicine |
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| Keywords | TSH, radioreceptor assay, TSH-binding inhibitor immunoglobulins, Graves' disease |
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