| Japanese |
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| Title | Dextran-coated charcoal法によるB・F分離に及ぼす遊離脂肪酸の影響について |
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| Subtitle | 原著 |
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| Authors | 多田正己*, 坂本健一*, 岡本晃*, 猪尾和弘*, 宮村敬*, 池田裕** |
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| Organization | *和歌山県立医科大学内分泌内科, **中央検査室 |
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| Journal | 核医学 |
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| Volume | 20 |
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| Number | 2 |
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| Page | 135-140 |
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| Year/Month | 1983/3 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」日常臨床に広く利用されているradioimmunoassay (RIA) の結合型 (B) と遊離型 (F) の分離には多くの方法が利用されているが, 吸着法の一つであるdextran-coated charcoal法 (D.C.C.法) において, 分離の際生じるB/T比の上昇要因について検討した結果, 遊離脂肪酸がD.C.C.による遊離型標識ステロイド吸着を阻害することにより, B/T比の上昇をきたし, 測定値としてはunderestimateされることになることをestriol (E3) のRIAについて明らかにした. B・F分離にD.C.C.を利用したステロイド物質のRIAでは, この事実を考慮する必要がある. |
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| Practice | 臨床医学：一般 |
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| Keywords | Dextran-coated charcoal, the separation of 'bound' from 'free' hormone (B・F separation), Free fatty acids |
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| English |
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| Title | Effect of Free Fatty Acids on the Separation of Bound from Free Hormone by Dextran-coated Charcoal Method |
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| Subtitle | Original Articles |
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| Authors | Masami TADA*, Kenichi SAKAMOTO*, Akira OKAMOTO*, Kazuhiro IWO*, Kei MIYAMURA*, Hiroshi IKEDA** |
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| Authors(kana) | |
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| Organization | *Department of Internal Medicine, Division of Endocrinology, Wakayama Medical School, **Department of Clinical Laboratory, Wakayama Medical School |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 20 |
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| Number | 2 |
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| Page | 135-140 |
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| Year/Month | 1983/3 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] In a radioimmunoassay utilizing dextran-coated charcoal as an adsorbent of unbound hormone after the incubation, addition of free fatty acids caused an apparent increase in antibody-bound estriol (E3) present in the supernatant fraction of reaction mixture after the final centrifugation in the assay procedure. Such unwarranted interference by free fatty acids was shown to occur at the adsorption step of unbound E3 by the dextran-coated charcoal, and the radioactivity due to erroneously remaining E3 in the bound fraction was shown to results in false elevation of "bound"/"total" ratio, and hence, underestimation of E3 concentration. Influence of free fatty acids on the determination of E3 and presumably other steroid hormones should be taken into consideration for radioimmunoassays in which dextran-coated charcoal is to serve as an adsorbent of the unbound moieties. |
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| Practice | Clinical medicine |
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| Keywords | Dextran-coated charcoal, the separation of 'bound' from 'free' hormone (B・F separation), Free fatty acids |
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