| Japanese |
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| Title | 血中癌胎児性抗原 (CEA) の新しい測定法の検討 - CEAキット「第一」の基礎的検討とその臨床応用 - |
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| Subtitle | 原著 |
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| Authors | 松岡雄治*, 黒木政秀*, 古賀芳子*, 王丸明子**, 宮内貞一**, 小野庸** |
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| Authors(kana) | |
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| Organization | *福岡大学医学部第一生化学, **放射線科 |
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| Journal | 核医学 |
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| Volume | 19 |
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| Number | 7 |
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| Page | 1023-1033 |
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| Year/Month | 1982/8 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」 今回, 第一ラジオアイソトープ研究所で作られた新しい血中CEA測定キットにつき, 基礎的事項を検討した. 1) 測定系は固相抗体sandwich法で, 50μl の血清を用い, 抽出も遠心操作も必要としない簡単な直接法で, 測定範囲は 1〜300ng/ml である. 2) 精度, 再現性, 希釈試験, 回収試験の結果は良好で, 臨床検査法として十分使用に耐える. 正常成人血清の平均値は2.5±1.0ng/ml. 3) Roche社, Dainabot社および本法の3者による臨床例の測定値を比較したところ, Rocheの値が最も高く, Dainabotはその1/10以下で本法の値は両者のほぼ中間 (Rocheの1/3強) に位置し, 両者のいずれともよく相関していた. 4) 上記3法により臨床経過を観察した例では, 本法の値はRocheの値と平行した変化を示したが, Dainabotの値は低く変動が把握しにくい. 以上の結果に基づいて現行CEA測定法の問題点を考察した. |
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| Practice | 臨床医学：一般 |
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| Keywords | carcinoembryonic antigen (CEA), radioimmunoassay, direct method |
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| English |
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| Title | Studies on a New Direct Solid-Phase Radioimmunoassay for Carcinoembryonic Antigen - Some Fundamental Studies on CEA kit "Daiichi" and its Clinical Application - |
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| Subtitle | Original Articles |
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| Authors | Yuji MATSUOKA*, Masahide KUROKI*, Yoshiko KOGA*, Akiko OHMARU**, Teiichi MIYAUCHI**, Yoh ONO** |
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| Authors(kana) | |
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| Organization | *First Department of Biochemistry, **Department of Radiology, School of Medicine, Fukuoka University |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 19 |
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| Number | 7 |
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| Page | 1023-1033 |
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| Year/Month | 1982/8 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] We evaluated a new radioimmunoassay kit for CEA (CEA kit "Daiichi") established by Daiichi Radioisotope Laboratories, Ltd. (Tokyo). 1. The kit is based on a solid-phase sandwich method using antibody-coated plastic beads and needs neither pretreatment of serum or plasma samples nor centrifugation in all steps. Only 50μl of sample is enough for the determination, and the assay range is 1 to 300ng/ml. 2. The accuracy, reproducibility, dilution and recovery tests proved the reliability of the kit. The mean value+-S.D. for 48 healthy adults was 2.5+-1.0ng/ml. 3. We compared the results obtained by this kit for a group of pathological serum samples with those by both Z-gel method (Hoffmann-La Roche) and antibody-coated paper disc method (Dainabot). The values obtained by Roche kit were generally the highest and those by Dainabot kit were the lowest. The values obtained by CEA kit "Daiichi" were found to be medium and correlated well with both values. 4. Follow-up studies of several patients indicated that the values by this kit fluctuated in parallel with those by Roche kit, but the Dainabot kit were less sensitive for the detailed analysis of the increase or decrease of CEA levels. |
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| Practice | Clinical medicine |
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| Keywords | carcinoembryonic antigen (CEA), radioimmunoassay, direct method |
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