| Japanese |
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| Title | 癌胎児性抗原CEAのisomeric speciesの分離精製とそのradioimmunoassayの臨床的評価 - 第一編 CEAのisomeric speciesの分離精製 - |
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| Subtitle | 原著 |
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| Authors | 吉井正雄* |
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| Authors(kana) | |
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| Organization | *京都大学医学部附属病院放射線科 |
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| Journal | 核医学 |
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| Volume | 19 |
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| Number | 3 |
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| Page | 375-385 |
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| Year/Month | 1982/4 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」大腸癌肝転移病巣より, 過塩素酸抽出, sepharose 4Bゲル濾過, DEAEセルロースカラムクロマトグラフィー, sepharose G-200ゲル濾過, 等電点電気分画法および超遠心分画法によってCEAのsingle isomer(CEA-S)を分離精製した. このCEA-Sは従来のheterogenousなCEAのうち, 等電点電気分画法によってpI 4.5の分画を採り, 超遠心分画法によって密度1.410 g/dlに相当する分画を採ったCEAのisomerであり, 分子量は約185000であった. このCEA-Sのアミノ酸組成はPlowおよびEdgingtonのCEA-Sと近似していた. また糖組成は全重量の27.7%で, 従来のCEAの糖組成より著しく少なく, sialic acidやN-acetyl galactosamineは検出されなかった. このCEA-Sは, 石川のCEAおよびGoldのCEAに比べてCEA活性が約1/4〜1/6であった. 以上の結果より, このCEA-SはPlowおよびEdgingtonのCEA-Sに近いCEAのisomerであると考えられた. |
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| Practice | 臨床医学：一般 |
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| Keywords | CEA, CEA-S, Heterogeneity of CEA. |
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| English |
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| Title | I Isolation of an Isomeric Species of Carcinoembryonic Antigen (CEA) : CEA - S |
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| Authors | Masao YOSHII |
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| Authors(kana) | |
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| Organization | Department of Radiology and Nuclear Medicine, Kyoto University, School of Medicine |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 19 |
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| Number | 3 |
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| Page | 375-385 |
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| Year/Month | 1982/4 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | A single isomeric species of carcinoembryonic antigen was isolated by extraction of tumor tissue of metastatic liver cancer from colon adenocarcinoma, with 1 M perchloric acid(PCA)followed by Sepharose 4B gel filtration, DEAE cellulose chromatography, Sephadex G-200 gel filtration, isoelectric focusing and isopyknic density gradient ultracentrifugation. The final product, representing approximately 0.31% of the PCA soluble glycoprotein of the tumor, showed a single discrete band in SDS polyacrylamide gel electrophoresis and was estimated to have a molecular weight of about 185000. This single species of CEA, CEA-S represent a density of 1.41 mg/g in cesium chloride and an isoelectric point(pI)of 4.5 of heterogenous conventional CEA. Amino acid compositions of the CEA-S share 27.7% in weight of total weight and are lack of sialic acid and N-acetyl galactosamine. Competitive inhibition analyse demonstrated the CEA-S had about one-fifth of reactivity to anti CEA antiserum compared to conventional CEA. Thus the CEA-S is considered to represent an isomeric species of CEA and to be similar to Plow's CEA. S. |
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| Practice | Clinical medicine |
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| Keywords | CEA, CEA-S, Heterogeneity of CEA. |
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