| Japanese |
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| Title | 111In-oxineによる血小板標識の基礎的検討 |
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| Subtitle | 原著 |
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| Authors | 油井徳雄*, 内田立身*, 松田信*, 室井秀一*, 田中鉄五郎*, 斎藤勝**, 刈米重夫* |
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| Organization | *福島県立医科大学第1内科, **福島県立医科大学RI研究室 |
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| Journal | 核医学 |
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| Volume | 18 |
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| Number | 4 |
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| Page | 463-472 |
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| Year/Month | 1981/5 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | <要旨> 111In-oxineによる血小板標識の基礎的検討を行い, 血小板寿命測定および血栓の描出等の臨床応用のための標識法を確立した. 111In-oxineにより血小板を, 1)button ACD-saline法, 2)buttonless ACD-saline法, 3)button ACD-saline Plasma法, 4)button plasma法の4方法で標識し, 標識の際の諸条件について検討した. その結果標識率は, インキュベーション時間, 温度, 血小板浮遊液中の血小板数, トランスフェリン濃度およびpHの影響を受けた. ラットで血小板寿命測定を行った結果, 血小板寿命には各々の方法の間に差異を認めなかったが, 回収率は遠心回数の多い1)法のみが低値を示した. 標識血小板の臓器分布の観察でも1)法のみに肝および脾に他の方法よりも有意に高い放射能を認めた. 以上の基礎的検討の結果, 血漿中のトランスフェリンの影響を少なくするためACD-saline液に血小板を浮遊させて標識する3)法は, 操作が簡便でかつ標識率も62 ± 5%と良好であるため臨床応用には最適と考えた. |
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| Practice | 臨床医学：一般 |
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| Keywords | Platelet labeling, In-111-oxine, Cr-51, Platelet survival, Rat |
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| English |
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| Title | Basic Study of Platelet Labeling with 111In-oxine |
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| Subtitle | Original Articles |
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| Authors | Tokuo YUI*, Tatsumi UCHIDA*, Shin MATSUDA*, Shuichi MUROI*, Tetsugoro TANAKA*, Masaru SAITO**, Shigeo KARIYONE* |
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| Organization | *The First Department of Internal Medicine, and **Radioisotope Laboratory, Fukushima Medical College |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 18 |
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| Number | 4 |
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| Page | 463-472 |
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| Year/Month | 1981/5 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] Indium-111-oxine has recently been suggested as a new isotopic labeling agent of platelets. In this paper, the results on the investigation of in vitro labeling of human platelets with In-111-oxine and those of platelet kinetics in rats are presented. Based on the findings of those studies, the protocol of human platelet labeling with In-111-oxine for clinical use was established. All operations should be carried out with sterile techniques at 20-25°C. 1)Fourty four ml venous blood is drawn into a 50ml polystyrene syringe containing 6 ml ACD-A. 2)The blood is transferred to a 50 ml tube and centrifuged at 300 g for 15 min. 3)Supernatant platelet rich plasma(PRP)is transferred to other 50 ml tube. Then, the pH is adjusted to 6.5 by addition of 1ml ACD-A per 20 ml PRP. 4)Platelets are sedimented by centrifuging at 1,500g for 15min and resuspended in 3 ml ACD-A-saline solution(pH 6.5). 5)Three hundreds μCi of In-111-oxine is added to the platelet suspension. The mixture is incubated for 20 min at room temperatur. 6)About 15 ml of the platelet poor autologous plasma(PPP)is added into the incubated mixture, followed by the sedimentation of labeled platelets(1,500 g, 15 min). 7)The labeled platelets are suspended in 10 ml PPP and the contaminating red cells are sedimented by centrifuging at 200g for 5 min. 8)One hundred and fifty μCi of labeled platelet suspension is injected to the patient intravenously. The labeling efficiency in this method was 62 +- 5%(mean +- 1S.D. , n = 6). |
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| Practice | Clinical medicine |
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| Keywords | Platelet labeling, In-111-oxine, Cr-51, Platelet survival, Rat |
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