| Japanese |
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| Title | 肝・胆道スキャン用剤の人血清蛋白結合に関する電気泳動法による検討 |
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| Subtitle | 《原著》 |
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| Authors | 伊藤和夫* |
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| Authors(kana) | |
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| Organization | *北海道大学医学部放射線科 |
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| Journal | 核医学 |
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| Volume | 17 |
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| Number | 8 |
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| Page | 921-928 |
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| Year/Month | 1980/9 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」99mTc標識化合物4種類(99mTc-DM-IDA, 99mTc-DE-IDA, 99mTc-PI, 99mTc-PB-IDA)と131I標識化合物2種類(131I-BSP, 131I-RB)の正常人血清蛋白に対する結合に関して, 電気泳動法による検討を行なった. 99mTc標識化合物は, すべて99mTc-HSAよりも陽極側に移動するが, 人血清蛋白に対する結合はすべて乏しい展開パターンしか示さなかった. その反面, 131I-BSPと131I-RBは, 人血清蛋白・アルブミンとほぼ100%結合した展開パターンを示した. この131I-BSPと131I-RBに対して, 非標識BSPと血清ビリルビンの影響について検討した. 131I-BSPは非放射性BSPの負荷により非蛋白結合部分の増加が観察されたが, 131I-RBは影響を受けなかった. 血清ビリルビン値22 mg/dlの人血清混和後の展開では, 131I-BSPは非蛋白結合部分の増加を認めなかったが, 131I-RBはその割合が増加することが観察された. |
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| Practice | 臨床医学：一般 |
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| Keywords | Hepatobiliary radio-agents, Protein binding, Electrophoresis, Hepatobiliary scan |
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| English |
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| Title | Electrophoretic Studies on Serum Protein Binding of Radiopharmaceuticals for Hepatobiliary Scan |
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| Subtitle | |
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| Authors | Kazuo ITOH |
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| Authors(kana) | |
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| Organization | Department of Radiology, Hokkaido University Hospital |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 17 |
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| Number | 8 |
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| Page | 921-928 |
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| Year/Month | 1980/9 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] Protein binding analyses of verious radiocompounds for hepatobiliary scintigraphy were performed on electrophoresis using a cellulose acetate menbrane in 0.05 M, pH 8.5 barbital buffer solution. The menbranes obtained were exposed tightly to X-ray film for making antoradiograms which were followed by a Microphotometry for semi-quantitative analysis. All of four 99mTc-labeled hepatobiliary agents, 99mTc-DM-IDA, 99mTC-DE-IDA, 99mTc-PI and 99mTc-PB-IDA, migrated to an anode, at the further distance than that of 99mTc-HSA employed as a control. These radioagents showed poor affinity to the albumin in the serum proteins. The other hand,131I-labeled agents, 131I-BSP and 131I-RB, represented a very good binding to the human serum albumin. In further analysis, very interesting phenomena were shown in protein binding of 131I-BSP and 131I-RB. There was a increase of unbound compornent of 131I-BSP noted after adding of non-radioactive BSP to the human serum mixture with it. However, 131I-RB was not affected in it's protein affinity by adding of non-radioactive BSP. The other hand, hyperbilirubinemia (22 mg/dl) affected a protein affinity of 131I-RB to serum albumin but gave no significant affection to that of 131I-BSP. These results suggest that radioagents for hepatobiliary scan are not only qualitative but also quantitative, specific and competitive in protein binding to a human serum albumin. |
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| Practice | Clinical medicine |
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| Keywords | Hepatobiliary radio-agents, Protein binding, Electrophoresis, Hepatobiliary scan |
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