| Japanese |
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| Title | L-3, 3'-diiodothyronineのRadioimmunoassay |
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| Subtitle | 原著 |
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| Authors | 高木淳*, 礒崎陽子*, 倉田邦夫*, 長滝重信** |
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| Organization | *ダイナボットRI研究所, **東京大学医学部附属病院第3内科 |
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| Journal | 核医学 |
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| Volume | 17 |
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| Number | 3 |
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| Page | 311-315 |
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| Year/Month | 1980/4 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」血中3,3'-diiodothyronine (3,3'-T2) のRadioimmunoassayによる測定法を確立した. 抗3,3'-T2血清は, L-3,3'-T2を, カーボデイミドを用いて, 牛血清アルブミンに結合し, complete Freund's ajuvantと共に家兎に感作して作製した. 得られた抗血清は, 甲状腺ホルモンおよびその誘導体とは免疫交叉性を示さきわめて特異的であった. 3,3'-T2-125Iは, 3-monoiodothyronineより, クロラミンT法で作製し, 比放射ず, 能3,000 μCi/μgのものを得た. 本法における, 最大検出感度は0.5 ng/100 mlであった. この方法で測定した正常人血中3,3'-T2は, 平均3.6 ng/100 ml (n=29) であり, 甲状腺機能亢進症で高値, 甲状腺機能低下症で低値であった. ウサギに, T3またはrT3を投与した時の血中3,3'-T2を測定すると, rT3を投与した時のみ血中に, 3,3'-T2が出現し, 3,3'-T2は, 主に, rT3の代謝によって産生されると推察された. |
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| Practice | 臨床医学：一般 |
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| Keywords | 3,3',5'-triiodothyronine rT3, 3,3'-diiodothyronine 3, 3'-T2, 3-monoiodothyronine 3-T1, 3,3',5-triiodothyronine T3, Thyroxine T4. |
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| English |
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| Title | Radioimmunoassay for 3,3'-diiodothyronine |
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| Subtitle | Original Articles |
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| Authors | Atsushi TAKAGI*, Yoko ISOZAKI*, Kunio KURATA*, Shigenobu NAGATAKI** |
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| Authors(kana) | |
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| Organization | *Dainabot Radioisotope Laboratories, Ltd., **The Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 17 |
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| Number | 3 |
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| Page | 311-315 |
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| Year/Month | 1980/4 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] A sensitive, specific and reproducible radioimmunoassay (RIA) for the measurement of 3,3'-diiodothyronine (3,3'-T2) in serum is described. A highly specific 3,3'-T2 binding antiserum was prepared by immunization of rabbits with 3,3'-T2-bovine serum albumin conjugate. A method for the preparation of 125I-3,3'-T2 of very high specific activity which was iodinated from 3-monoiodothyronine was developed. Utilizing this RIA, a mean serum 3,3'-T2 level of normal subjects was 3.60+-1.5 ng/100 ml (mean+-SD, n=29) . Serum 3,3'-T2 was found to be increased in hyperthyroidism, and was decreased in hypothyroidism. Serum 3,3'-T2 concentrations after iv injection of T3 or rT3 in rabbits increased sharply after injection of rT3, but did not increased after T3 injection. These results suggest that rT3 is the major source of 3,3'-T2 in the serum. |
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| Practice | Clinical medicine |
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| Keywords | 3,3',5'-triiodothyronine rT3, 3,3'-diiodothyronine 3, 3'-T2, 3-monoiodothyronine 3-T1, 3,3',5-triiodothyronine T3, Thyroxine T4. |
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