| Japanese |
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| Title | 111In-oxineによるリンパ球標識の基礎的検討と, マウスおよびラットにおけるリンパ球回転への応用 |
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| Subtitle | 原著 |
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| Authors | 松田信*, 内田立身*, 国分令子*, 油井徳雄*, 木村秀夫*, 田中鉄五郎*, 秋月健*, 刈米重夫*, 斎藤勝** |
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| Authors(kana) | |
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| Organization | *福島県立医科大学第1内科, **福島県立医科大学RI研究室 |
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| Journal | 核医学 |
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| Volume | 16 |
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| Number | 8 |
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| Page | 1373-1378 |
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| Year/Month | 1979/11 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」111In-oxineによるヒトリンパ球標識法と, 111In-oxine標識リンパ球を用いたリンパ球回転を, マウスとラットで検討した. リンパ球標識はThakurらの方法に準じて行なった. 111In-oxineによるリンパ球標識率は37℃, 20分で34.3±6.0%であり, Trypan blue排除試験によるviabilityは93.3±1.8%であった. マウスにおいて標識リンパ球の血中消失曲線は2相性に減少し, 第1相の半減期は0.51時間, 第2相の半減期は38.9時間であった. 臓器分布の検討では, 肺, 肝, 脾が最も関与していた. またラットにおいてシンチレーションカメラで, 臓器分布を観察することが可能であることを示した. 111In-oxine標識リンパ球を用いた場合の臓器分布は, 先に私たちが報告した51Cr, 99mTcを用いた場合と同じ傾向を示した. 111In-oxineは効率良く, ヒトおよびマウスなどのリンパ球を標識することができ, 細胞障害作用もなく, リンパ球回転の基礎的, 臨床的応用に非常に有用であると考えられた. |
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| Practice | 臨床医学：一般 |
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| Keywords | 111In-oxine, lymphocytes, lymphocyte kinetics |
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| English |
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| Title | Fundamental Studies of Lymphocyte Labeling with in-111-oxine and It's Application to Lymphocyte Kinetics Mice and Rats |
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| Subtitle | Original Articles |
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| Authors | Shin MATSUDA*, Tatsumi UCHIDA*, Reiko KOKUBUN*, Tokuo YUI*, Hideo KIMURA*, Testugoro TANAKA*, Tsuyoshi AKITSUKI*, Masaru SAITO**, Shigeo KARIYONE* |
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| Authors(kana) | |
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| Organization | *First Department of Internal Medicine and **Radioisotope Laboratory, Fukushima Medical College |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 16 |
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| Number | 8 |
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| Page | 1373-1378 |
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| Year/Month | 1979/11 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] Fundamental studies of lymphocyte labeling with In-111-oxine and it's application to lymphocyte kinetics in mice and rats were studied. Lymphocyte labeling with In-111-oxine was performed by using the method of Thakur et al (1976). In order to extract In-111-oxine complex efficiently, the solution was adjusted to pH 5 to 6, and labeling efficiency of lymphocytes was 34.3+-6.0% at 37℃ for 20 minutes. Cell viability evaluated by supervital staining with trypan blue was 93.3+-1.8%. Two times washings were sufficient to remove the radioactivity of free In-111-oxine. In the mice, disappearance curve of In-111-oxine labeled lymphocytes from blood stream showed two exponential components. T 1/2 of the first component was 0.51 hr and that of the second one 38.9 hr, respectively. In the studies of organ distribution, labeled lymphocytes accumulated to lungs, liver and spleen which was similar to that with Cr-51 and Tc-99m. By using the scintillation camera, organ distribution of In-111-oxine labeled lymphocytes was visualized in the rat 30 minutes after infusion. It was discussed that In-111-oxine is quite useful radioisotope for labeling lymphocytes, and it's application to animal or human beings would overcome the difficulty of lymphocyte kinetics and organ distribution by previous Cr-51 or Tc-99m. |
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| Practice | Clinical medicine |
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| Keywords | 111In-oxine, lymphocytes, lymphocyte kinetics |
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