| Japanese |
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| Title | 99mTcによる血球標識にかんする研究 (第4報) - 99mTcによるリンパ球標識の基礎的検討 - |
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| Subtitle | 原著 |
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| Authors | 松田信*, 内田立身*, 油井徳雄*, 三浦信雄*, 木村秀夫*, 田中鉄五郎*, 秋月健*, 吉田博*, 刈米重夫*, 斎藤勝**, 木田利之*** |
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| Authors(kana) | |
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| Organization | *福島県立医科大学第1内科教室, **福島県立医科大学RI研究室, ***福島県立医科大学放射線科 |
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| Journal | 核医学 |
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| Volume | 16 |
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| Number | 3 |
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| Page | 301-307 |
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| Year/Month | 1979/5 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要旨」99mTcによるリンパ球標識を, CIS製赤血球標識用キットを用いて検討した. 標識率は5.3±0.4% (mean±1 S.D., n=8) であり, トリパンブルー排除試験によるviabilityは92.1±2.3% (n=3) であった. 連続洗浄によるリンパ球標識率の変動の観察では, 遊離99mTcをとり除くのに3回の洗浄が必要であった. 以上より本標識法はBarthらの方法に優るとも劣らぬ方法であると思われた. マウスにおける実験では, 標識リンパ球は2相性に指数関数的に減少した. 第1相のT1/2は0.5時間, 第2相のT1/2は6.1時間であった. 臓器分布の検討では, 注入直後, 肺に高い放射能を認めたが, 経時的に減少した. 脾・肝臓の放射能は注入後1時間まで増加し, その後プラトーに達した. これらのリンパ球回転の成績は, 51Crを用いた実験と同じ結果を示した. 真性多血症患者において, 99mTc標識リンパの臓器分布をガンマーカメラで観察可能な事を示した. 注入30分後, 脾臓に放射能の集積が認められた. 以上より, 99mTcはリンパ球回転の基礎的, 臨床的研究に適したものと考えられた. |
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| Practice | 臨床医学：一般 |
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| Keywords | 99mTc, lymphocyte, lymphocyte kinetics, Organdistribution |
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| English |
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| Title | Blood Cell Labeling with Technetium-99m (IV) Studies of Lymphocyte Labeling by 99mTc |
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| Subtitle | Original Articles |
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| Authors | Shin MATSUDA*, Tatsumi UCHIDA*, Tokuo YUI*, Nobuo MIURA*, Hideo KIMURA*, Tetsugoro TANAKA*, Tsuyoshi AKITSUKI*, Hiroshi YOSHIDA*, Shigeo KARIYONE*, Masaru SAITO**, Toshiyuki KIDA*** |
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| Authors(kana) | |
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| Organization | *The First Department of Internal Medicine, **Radioisotope Laboratory, and ***Department of Radiology, Fukushima Medical College |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 16 |
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| Number | 3 |
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| Page | 301-307 |
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| Year/Month | 1979/5 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] Lymphocytes were labeled with 99mTc using CIS kit for labeling red blood cells. Labeling efficiency was 5.3+-0.4% (mean+-1 S.D., n=8) . Viability was evaluated by supervital staining with trypan blue and its value was 92.1+-2.3% (n=3) . Repeated washing of labeled lymphocytes were performed in order to observe the changes of rates of 99mTc activity from the labeled cells. Free 99mTc was completely removed after washing three times. From these results, this labeling method was concidered to be better than that of Barth et al. already described. In the mice, disappearance curve of 99mTc labeled lymphocytes from blood stream showed two exponential components. T1/2 of the first component was 0.5 hr and T1/2 of the second one 6.1 hr, respectively. Studies of organ distribution were performed in C3H mice. Immediately after the infusion of the labeled lymphocytes, high radioactivity was detected in the lung and its radioactivity decreased rapidly in the course of time. The radioactivity of the spleen and the liver increased until 1 hr after infusion and achieved plateau. The organ distriubtion of 99mTc labeled lymphocytes was similar to that observed with 51Cr. In a patient with polycytemia vera, scintiphotogram by gammer camera after infusion of 99mTc labeled lymphocytes was obtained. The accumulation of radioactivity on the spleen was detected at 30 min. after infusion. 99mTc can be labeled to mice and human lymphocytes efficiently without any damages of the cells. 99mTc labeled lymphocytes is quite useful for experimental and clinical studies of lymphocyte kinetics. |
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| Practice | Clinical medicine |
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| Keywords | 99mTc, lymphocyte, lymphocyte kinetics, Organdistribution |
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