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| Title | A Radioimmunoassay for Measurement of 3, 3', 5', -Triiodothyronine (Reverse T3) |
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| Authors | Atsushi TAKAGI, Yoko ISOZAKI, Kunio KURATA, Shigenobu NAGATAKI* |
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| Organization | Dainabot Radioisotope Laboratories, Ltd., *The Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 15 |
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| Number | 2 |
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| Page | 275-281 |
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| Year/Month | 1978/4 |
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| Article | Report |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [ABSTRACT]A sensitive, specific and reproducible radioimmuoassay (RIA) for the measurement of 3, 3' 5'-triiodothyronine (rT3) in serum without extraction is described. A highly specific rT3 binding antiserum was prepared by immunization of rabbits with rT3-bovine serum albumin conjugate A method for the preparation of 125I-rT3 of very high specific activity which was iodinated from 3-monoiodothyronine was developed. Utilizing this RIA, a mean (+-SE) serum rT3 level of normal subject was 27.9+-0.9 ng/100 ml (n=59). Serum rT3 was found to be increased in hyperthyroidism (168.1+-13.8 ng/100 ml, n=13), normal pregnancy (36.4+-2.2 ng/100 ml, n=10), complete starvation (58.0+-9.8 ng/100 ml, n=3), anorexia nervosa (41.6+-4.8 ng/100ml, n=30). And the serum concentration was decreased in hypothyroidism (9.8+-1.0 ng/100ml, n=10). The method described here satisfies requirments for measurement of rT3 by RIA. |
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| Practice | Clinical medicine |
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| Keywords | Radioimmunoassay, Reverse T3, 125I-rT3, Polyethylene glycol(PEG), 8-anilino-1-naphthalen-sulfonic acid(ANS) |
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| Japanese |
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| Title | 3', 3', 5'-トリヨードチロニン (rT3) のラジオイムノアッセイ |
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| Subtitle | ノート |
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| Authors | 高木淳, 礒崎陽子, 倉田邦夫, 長滝重信* |
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| Organization | (株)ダイナボットラジオアイソトープ研究所, *東京大学医学部第三内科 |
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| Journal | 核医学 |
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| Volume | 15 |
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| Number | 2 |
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| Page | 275-281 |
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| Year/Month | 1978/4 |
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| Article | 報告 |
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| Publisher | 日本核医学会 |
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| Abstract | 血中3, 3', 5'-トリヨードチロニン(rT3)のラジオイムノアッセイ(RIA)による測定法を検討した. 抗rT3抗体は, ウシ血清アルブミンと結合させたrT3を, 家兎に投与して得た. 3, 5, 3'-トリヨードチロニン(T3)及びチロキシン(T4)との交叉反応は, それぞれ, 0.0001%以下, 0.009%であり, 本法による, rT3測定値は, T3及びT4の影響をほとんど受けていないと考えられる. 125I-rT3は, 3-モノヨードチロニンをクロラミンT法で標識し, 4,500〜5,500μCi/μgの高比放射能を得た. 本法の測定によって得た正常者59人の血中rT3値は, 27.9±0.9ng/100ml(Mean±SE)であった. 又, 甲状腺機能亢進症, 正常妊婦, 絶食者および, 神経性食思不振症の血中rT3値も, 正常者に比較し, 高値を示し, 甲状腺機能低下症において, 正常より低値を示した. |
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| Practice | 臨床医学：一般 |
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| Keywords | Radioimmunoassay, Reverse T3, 125I-rT3, Polyethylene glycol(PEG), 8-anilino-1-naphthalen-sulfonic acid(ANS) |
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