| Japanese |
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| Title | ヒト肝フェリチンのRadioimmunoassayに関する基礎的研究 |
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| Subtitle | 原著 |
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| Authors | 中治隆宏*, 馬場茂明** |
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| Organization | *神戸大学医学部内科学第2講座 **指導:教授 |
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| Journal | 核医学 |
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| Volume | 14 |
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| Number | 6 |
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| Page | 887-896 |
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| Year/Month | 1977/12 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「I. はじめに」フェリチンは鉄貯蔵蛋白質として組織中に認められる蛋白質であり, 哺乳動物の肝臓, 脾臓, 骨髄, 腎臓, 心臓, 小腸粘膜, 骨格筋, 胎盤などあらゆる組織中に見出され, その分子形態は蛋白の殻の中に鉄ミセルを持つとされている. その分子量は44万〜48万であり, その分子は20〜24個の均一なsubunitから成りたっているといわれている. 血中でのフェリチンの検出は1956年, Reissman & Dietrichにはじまるが, 彼等は血中へのフェリチンの出現は肝細胞死によって, フェリチンが血中へ逸脱してくると考えた. しかし, その後の研究によって肝障害患者のみならず, 種々の疾患 (白血病, 再生不良性貧血, 悪性リンパ腫など) で血中フェリチンの増加することが報告されるとともに, 高感度の免疫放射測定法の進歩によって病的患者血清のみならず, 健常人血清中でも測定することが可能となった. 著者はフェリチンが肝細胞の細胞分画法においてマイクロゾーム分画に大量に含まれること, またこの蛋白が免疫電気泳動上α2位にくることを利用して超遠沈による細胞分画法, 電気泳動法, ゲル濾過法を組み合わせてヒト肝フェリチンの分離精製を試みた. |
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| Practice | 臨床医学：一般 |
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| English |
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| Title | Studies on a Double Antibody Radioimmunoassay for the Determination of Human Ferritin |
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| Subtitle | Original articles |
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| Authors | Takahiro NAKAJI, Shigeaki, Baba* |
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| Organization | The Second Department of Internal Medicine, Kobe University School of Medicine, *Director |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 14 |
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| Number | 6 |
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| Page | 887-896 |
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| Year/Month | 1977/12 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] : A double antibody radioimmunoassay method for the determination of human liver ferritin was studied to establish its validity of microassay. We purified serum protein-free ferritin from human livers, and made anti-human liver ferritin rabbit serum by immunizing with the purified ferritin. A modified method of Hunter and Greenwood was used for the 125I-labelling of the ferritin. 125I-labeled ferritin was separated through a sephadex-G75 column and repurified through G-200 column before assay. The specific radioactivity of the labeled ferritin ranged from 0.3 to 0.4μCi of 125I-per microgram of protein. All dilutions were made with 1/15 M phosphate buffered saline, PH 7.5, containing 1% bovine serum albumin (1% BSA-PBS) . One hundred μl of serum sample or standard ferritin was added to 100μl of anti-human liver ferritin rabbit serum (1 : 8000) and 500μl of buffer. Then, 125I-labeled ferritin (10,000 cpm=20 ng) was added. The solutions were mixed and incubated at 4°C for 36 hours. One hundred μl of the normal rabbit serum (1 : 50) and 100μl of the second antibody (1 : 10) were added to the solutions, followed by incubation at 4°C for 24 hours. The above assay conditions were found to be optimum, and the total and precipitated counts were measured by Packard auto-γ-counter. The standard ferritin was diluted in both the 1% BSA-PBS and the ferritin-free human serum obtained by sepharose 4B affinity chromatography using the anti-ferritin rabbit serum as ligand. The standard curves in these diluents were almost the same and showed that the ferritin-free human serum protein did not interfere with the assay system. The minimal ferritin concentration in normal serum detectable by this assay method was 31 ng/ml, and the mean value of ferritin in normal human serum was 177+--SD 103 ng/ml for male (36 samples) and 107+-SD 109 ng/ml for female (30 samples) , respectively. |
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| Practice | Clinical medicine |
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