Japanese
Title人成長ホルモンの簡易超微量定量法の開発とその臨床応用
Subtitle原著
Authors小川紀雄*, 高原二郎*, 大藤真*
Authors(kana)
Organization*岡山大学医学部第三内科 (主任 : 大藤真教授)
Journal核医学
Volume12
Number4
Page359-366
Year/Month1975/8
Article原著
Publisher日本核医学会
Abstract「1. はじめに」Radioimmunoassay (RIA) の開発以来10余年の間に血漿人成長ホルモン (human growth hormone : HGH) 値測定のために種々のRIAの変法が工夫され, かつまた用いられてきた. 現在用いられているすべてのRIAはincubation終了後に抗体結合型ホルモン (bound : B) と遊離型ホルモン (free : F) とを分離する必要があり, その目的のために2抗体法, クロマト電気泳動法, 塩析法, デキストラン炭末法その他多くの方法が用いられている. 固相法RIAはCattらによりHGHの測定法として最初に報告されたが, 水洗という簡単な手技のみでBとFの分離が可能な特異な方法であり, その後他のホルモンの血中濃度の測定に応用されている, 本稿は, 先に報告したplastic製microtiter trayを用いる固相法RIAを一部改変した, 血漿HGHの超微量定量法の開発に関するものである.
Practice臨床医学：一般
Keywords
English
TitleRoutine Ultramicro-Measurement of Human Growth Hormone in Plasma by Solid-Phase Radioimmunoassay and its Clinical Application
SubtitleOriginal Articles
AuthorsNorio OGAWA, Jiro TAKAHARA, Tadashi OFUJI
Authors(kana)
OrganizationThird Department of Internal Medicine, Okayama University Medical School
JournalThe Japanese Journal of nuclear medicine
Volume12
Number4
Page359-366
Year/Month1975/8
ArticleOriginal article
PublisherTHE JAPANESE SOCIETY OF NUCLEAR MEDICINE
Abstract[Summary] The development and application of the method for the ultramicro-measurement of human growth hormone (HGH) in plasma based on the solid-phase radioimmunoassay (RIA) by using antibody-coated disposable microtiter trays was reported, By further reduction in the amount of antibody with corresponding increase in the period of incubation, greater sensitivity has been achieved. 'This method' has adequate sensitivity and precision for routine ultramicro-measurements of plasma HGH concentration, and is relatively simple to perform The lower limit of sensitivity for purified HGH was 25pg/ml (P<0.05) . The precision of the measurement of HGH in plasma has been calculated, and the quantitative sensitivity of the method derived from the results given by its routine use was 56.8pg/ml (P<0.05) . There was no statistical significance between the besal HGH level obtained from this method and that from double-antibody RIA in normal subjects. On the other hand, the mean basal plasma HGH level after an overnight fasting in 21 hypopituitary patients was 484pg/ml (+-282 ; 1 SD) by this method, and this was significantly lower (P<0.001) than 1376pg/ml (+-498 ; 1 SD) by double-antibody RIA. These data show that determination of basal plasma HGH levels by this method is of much value in the diagnosis of hypopituitarism. Plasma HGH concentrations were determined by this method during standard oral 50g glucose tolerance tests in normal subjects, and there was a distinct decline but not reaching the sub-nanogram range. Thus high concentrations of blood glucose could not suppress completely HGH secretion in normal subjects.
PracticeClinical medicine
Keywords

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