Japanese
TitleRadioimmunoassayによる血漿レニン活性測定法の検討
Subtitle原著
Authors多川斉*, 前畑英介**, 石井当男***, 池田寿夫***
Authors(kana)
Organization*三井記念病院内科腎高血圧科, **中央検査部, ***東京大学第2内科
Journal核医学
Volume11
Number6
Page657-663
Year/Month1974/12
Article原著
Publisher日本核医学会
Abstract1969年Haberらがangiotensin Iのradioimmunoassayを利用した血漿レニン活性 (plasma renin activity ; PRA) の測定法を開発して以来, 本法やその改良法が広く使われ, 最近はキットが市販され, 従来のbioassayに代りつつある・しかし, その定量感度はなお満足できず, 特に本態性高血圧症における低レニン例の選別には不十分であると批判されている. 著者らは, さきにレニン・リアキット (ダイナボット) の使用経験を発表し, また従来から阿部らの方法を検討してきたが, 今回これらを若干modifyした方法を考察した. 本稿では, その方法を紹介し, あわせて本法とbioassay法, ならびにキットによる他のradioimmunoassay法とを比較報告する. 「方法」1) 125I 標識angietensin I, 抗体, および標準angietensin I 大阪大学蛋白質研究所ペプチド奨励会より供与されたangiotensin Iを用いた.
Practice臨床医学：一般
Keywords
English
TitleMeasurement of Plasma Renin Activity by Radioimmunoassay
SubtitleOriginal
AuthorsHitoshi TAGAWA, Eisuke MAEHATA, Masao ISHII, Toshio IKEDA
Authors(kana)
OrganizationDept. of Medicine and Central Clinical Laboratories, Mitsui Memorial Hospital, and 2nd Dept. of Medicine, Tokyo University Hospital
JournalThe Japanese Journal of nuclear medicine
Volume11
Number6
Page657-663
Year/Month1974/12
ArticleOriginal article
PublisherTHE JAPANESE SOCIETY OF NUCLEAR MEDICINE
Abstract[Summary]Modified method of radioimmunoassay of angiotensin I for measurement of plasma renin activity (PRA) was reported. Standard angiotensin I was pipetted to tubes containing tris acetate buffer (pH7.4), 125I-angiotensin I and antiserum (supplied by Dainabot RI Lab), and they were kept overnight at 4℃. Dextran-coated charcoal was used for separation of B and F. Standard curve was depicted by plotting the difference of B/T% between each angiotensin standard and 0-standard containing no angiotensin. In order to assay PRA, 5ml of blood was taken into a tube containing EDTA-Na2, and plasma was stored frozen. After thawing, the sample was divided to two, and was adjusted to pH 5.5. Diisopropyl fluorophosphate was added. One was 'incubated' for 3 hrs at 37℃, and another was kept at 4℃ ('unincubated'). Twenty microliter of the treated sample was taken and assayed in duplicate in the same way as angiotensin. Angiotensin I produced by incubation was calculated from the difference of B/T% between incubated and unincubated samples, which was expressed as PRA (ng/ml/hr). This procedure seemed to eliminate the possible error occurring especially in low-renin samples, which might be caused by the presence of factors in plasma. Both intra-and inter-assay variations were small. Recovery and dilution experiments gave excellent results. This method gave results correlated well with bioassay (r=0.905) and with two commercially available radioimmunoassay kits (r=0.941 and 0.971). In normotensive controls, PRA showed a tendency of inverse correlation with sodium excretion ; when sodium excretion was 50-200 mEq/day, however, PRA fell within a relatively small range, which was 1.10+-0.58ng/ml/hr (m+-SD) in the fasting state during recumbency. PRA nearly doubled by stimulation with either standing for an hour or IV injection of furosemide. We could not confirm consistent diurnal rhythm in 5 patients.
PracticeClinical medicine
Keywords

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