| Japanese |
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| Title | Radioimmunoassay法による血中Triiodothyronine濃度の測定 |
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| Subtitle | 原著 |
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| Authors | 伴良雄*, 井上健*, 児島孝典*, 長島則夫*, 渡辺恭行*, 宮本正浩*, 飯ヶ谷清*, 飯野史郎* |
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| Organization | *昭和大学医学部第三内科 |
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| Journal | 核医学 |
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| Volume | 11 |
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| Number | 4 |
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| Page | 479-487 |
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| Year/Month | 1974/8 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「緒言」ヒト血中甲状腺ホルモンには1-Thyroxine (T4)と1-Triiodothyronine (T3)があるが, T4に比し速効性でかつ生物学的活性の強いT3が甲状腺の機能状態や治療後の代謝状態を知る上で注目されていた. しかし血中T3は微量であるために最近に至るまでその化学的測定は不可能であったが, Sterlingら (1969) はcompetitive proteinbinding assay法によって, またHollander (1968) らはGas chromatography法によってそれぞれ血中T3濃度の測定に成功するに至った. これらの測定法はその信頼性において必ずしも満足すべきものではなかったが, 1959年BersonとYallowによりインスリンのRadioimmunoassay (RIA) 法が開発されて以来, 諸種の血中微量ホルモンの測定が可能となりその信頼性も極めて満足すべきものとなった. 今回, われわれも抗T3抗体を作成しT3のRIAに成功し, その基礎的ならびに臨床的検討を行ったのでその結果を報告する. |
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| Practice | 臨床医学：一般 |
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| English |
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| Title | A Study on the Radioimmunoassay of Serum Triiodothyronine |
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| Subtitle | Original |
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| Authors | Yoshio BAN, Takeshi INOUE, Takahumi KOJIMA, Norio NAGASHIMA, Yasuyuki WATANABE, Masahiro MIYAMOTO, Kiyoshi IIGAYA, Shiro IINO |
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| Authors(kana) | |
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| Organization | Third Department of Internal Medicine, Showa University School of Medicine |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 11 |
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| Number | 4 |
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| Page | 479-487 |
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| Year/Month | 1974/8 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary]Preparing antibody for triiodothyronine (T3) by ourselves, the serum T3 concentration were determined by radioimmunoassay. Twenty euthyroid normal control volunteers, twenty hyperthyroid patients, seven hypothyroid patients, three patients with subacute thyroiditis and one patient with TBG deficiency were employed in this stady. The conjugate of T3 to human serum albumin was made by carbodiimide Method of Oliver et al., emulsified in equivalent volume of complete Freund's adjuvant, then injected into back muscles of rabbit every week and sera were drawn and served for assay successively at adequate intervals. It was fonnd that the relative activity of thyroxine to T3 was 0.2%, but there is no effect on T3 concentration when 10 or 20 ng of thyroxine was added to standard doses of T3 or 1, 10, 20 or 100 ng of thyroxine to 150pg of T3, The relative activities of DIT and MIT to T3 were 0.009% and 0.008%, respectively. The assay procedure was similar to the conventional method, except for using T3 free serum treated with 8 -anilino-1-naphthalene sulfonic acid (ANS) and charcoal. The presision and the reproducibility of this assay system were quite excellent, having coefficient variation of 4.9% for the former, and 8.6% for the latter. The rate of recovery was 106%. The concentration of serum T8 was 127+-26 (mean+-S.D.) ng/ 100ml for euthyroid normal volunteers, 575+-262 ng/ 100ml for hyperthyroid patients and 54+-22ng / 100ml for hypothyroid patients. Our euthyroid average value was well comparable to those reported by Sakurada et al., Hachiya and Mitsuma. In three patients with subacute thyroiditis they were 120, 126 and 147ng / 100ml and in one patient with TBG deficiency it was 52ng / 100ml. It was concluded that the developed T8 radioimmunoassay system was very reliable and could be used for the routine T3 determination. |
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| Practice | Clinical medicine |
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