Japanese
Title放射性セレノメチオニンのマウス膵細胞の酸可溶性分画ならびにタンパク質分画へのとりこみについて
Subtitle原著
Authors五島廉輔*, 手塚雅勝*, 石上和子*, 為政脩*
Authors(kana)
Organization*静岡薬科大学放射薬品学教室
Journal核医学
Volume11
Number4
Page453-463
Year/Month1974/8
Article原著
Publisher日本核医学会
Abstract「1. はじめに」膵スキャンニング剤として, 75Se-セレノメチオニン (75Se-Met) が現在もっとも広く用いられている. 本物質は膵スキャンニング剤として応用する目的で, Blauによって1961年はじめて酵母を用いて生合成され, その翌年臨床実験に関する報告がなされた. 本物質の臓器内分布については, まず, Blau, Manskeによって検討され, 膵スキャンニング実施上防害臓器となる肝臓と比較して, 単位重量当たりでは膵臓によりよくとりこまれることが判明した. その後, Hanssonらはオートラジオグラフィーを用いる臓器中へのとりこみの比較ならびに膵臓以外の臓器中のタンパク質およびトリクロル酢酸可溶性分画 (酸可溶性分画) へのとりこみについて報告した. さらに水上は臨床学的目的を背景にして, 主として本物質の動物およびヒトでの血中および臓器内分布の経時的変化を検討した.
Practice臨床医学：一般
Keywords
English
TitleOn the Incorporation of 75Se-Selenomethionine into the Acid-Soluble and Protein Fractions of Mouse Pancreatic Cells
SubtitleOriginal
AuthorsRensllke GOTO, Masakatsu TEZUKA, Kazuko ISHIGAMI, Osamu TAMEMASA
Authors(kana)
OrganizationShizuoka Cellege of Pharmacy
JournalThe Japanese Journal of nuclear medicine
Volume11
Number4
Page453-463
Year/Month1974/8
ArticleOriginal article
PublisherTHE JAPANESE SOCIETY OF NUCLEAR MEDICINE
Abstract[Summary]In general, 75Se-selenomethionine (75Se-Met) is understood to be highly incorporated into enzyme proteins produced in the pancreas. However, because in some clinical applications the scanning is carried out within very short time (5-10min.) after injection, it was anticipated that such 75Se-Met incorporation into the pancreas within short time might be owing to the incorporation into acid-soluble fraction more than into protein fraction. From such a consideration, we worked out for a comparative study of 75Se-Met incorporation into acid-soluble and protein fractions of mouse pancreatic and liver cells, in vitro and in vivo. In vitro, the radioactivity of 75Se-Met incorporated into the acid-soluble fraction of normal mouse pancreatic cells was 1.5-2.5 times higher than that of protein fraction within 30 min., and the ratio of the liver cells was 2-9 within 90 min, The total incorporation (acid-soluble fraction + protein fraction) into pancreatic cells was about 3 times higher, through the incubation period within 90 min., than into liver cells. Fasting of mice had no effect on the incorporation. On the other hand, in vivo, the radioactivity of 75Se-Met in the acid-soluble fraction of normal mouse pancreatic cells was about one third of that in the protein fraction through the period after injection. However, it seems necessary in search for more useful pancreas-scanning agents to appreciate the significance of incorporation of natural and unnatural amino acids into acid-soluble fraction of pancreatic cells, because some unnatural amino acids were highly incorporated into the acid-soluble fraction. From this point of view, first, in order to find correlations among amino acids pool size in the acid-soluble fraction, their contents in the protein fraction and their incorporation into the both fractions, amino acid analysis of the both fractions of pancreatic and liver cells was carried out by a amino acid analyser. Consequently, the pool size of methionine, a mother substance of selenomethionine, was considerably small compared to that of other amino acids. Phenylalanine, a mother compound of iodophenylalalanines investigated as a pancreas-scanning agent, also had a small pool size. These results suggested that there might be a correlation between amino acids pool size and their incorporation into acid-soluble fraction of pancreatic cells. Furthermore, the effects of several energy sources, enzyme inhibitors and some natural amino acids on the in vitro incorporation of 75Se-Met were investigated. Among these substances, lactic acid, certain amino acids, 2,4-dinitrophenol and p-chloromercuribenzoate were more or less inhibitory on the incorporation.
PracticeClinical medicine
Keywords

【全文PDF】