Japanese
Titleガストリンのradioimmunoassayの検討
Subtitle原著
Authors本木達也, 加藤善久, 上井一男, 右田徹, 野村喜重郎, 佐々木康人, 原田尚, 亀田治男, 村尾覚
Authors(kana)
Organization東京大学 第2内科
Journal核医学
Volume10
Number4
Page373-379
Year/Month1973/8
Article原著
Publisher日本核医学会
Abstract「はじめに」胃の生理および病態の解明にガストリンに関する知見の発展は大いなる役割を果しつつある. 中でも1968年にMcGuiganによってはじめられたガストリンのradioimmunoassayは非常に有用な研究手段として短時日の間に世界の各施設で行なわれるに至った. わが国では谷内, 松尾をはじめとしてradioimmunoassayは数施設において行なわれるようになってきている. しかしわが国で今まで発表された本assay法の検討はいまだ理想的なレベルに達してなく, ガストリンのradioimmunoassayはむずかしいものの1つに数えられている. 抗ガストリン血清はわが国ではU.S.A. のWilson Laboratoriesの製品を使用しており, 同製品中低titer(1:5,000)の製品は安価ではあるが, 良好な標準曲線を得ることが困難であるといわれている. われわれはこのたび同社の(1:5,000)titer抗ガストリン血清を使用し2抗体法によるガストリンのradioimmunoassayを検討したのでその結果を報告する.
Practice臨床医学：一般
Keywords
English
TitleA study of radioimmunoassay of gastrin
SubtitleOriginal
AuthorsTatsuya MOTOKI, Yoshihisa KATO, Kazuo KAMII, Tohru MIGITA, Kijuro NOMURA, Yasuhito SASAKI, Takashi HARADA, Haruo KAMEDA, Satoru MURAO
Authors(kana)
Organization
JournalThe Japanese Journal of nuclear medicine
Volume10
Number4
Page373-379
Year/Month1973/8
ArticleOriginal article
PublisherTHE JAPANESE SOCIETY OF NUCLEAR MEDICINE
Abstract[Summary] A double antibody radioimmunoassay technique for measuring serum gastrin using 1:5,000 titer antigastrin serum of Wilson Laboratories was studied. Synthetic human gastrin I (SHG I) was labeled with 125I by a modification of the method of Hunter & Greenwood. Then, the labeled product was purified by gel filtration on Sephadex G-10 and G-50 columns. The specific activity of 125I-SHG, thus obtained, was 320-350μCi/μg. The assay method was as follows: (1) One-tenth ml of 125I-SHG (4,000cpm), 0.1ml of sample serum or standard SHG, 0.1ml of guinea pig antiserum to porcine gastrin (1:200) and 0.1ml of 0.15M NaCl-0.01M phosphate buffer (pH 7.4), containing 0.3% bovine serum albumin and 0.01M EDTA 2 Na, were mixed and incubated for 24 hours at 4℃. (2) After incubation, 0.2ml of rabbit antiserum to guinea pig γ-globulin (1:5) and 0.1ml of normal guinea pig serum (1:100) were added to the incubation mixture and incubated for further 24 hours at 4℃. After the second incubation, total radioactivity was counted by well-type scintillation counter and the mixture was centrifuged at 3,000rpm for 30 minutes at 4℃. The supernatant was removed and the radioactivity of the precipitate (bound form) was counted. Then B% was calculated. Standard diluent was 0.15M NaCl-0.01M phosphate buffer (pH 7.4) containing 0.3% bovine serum albumin and 0.01M EDTA 2 Na. A radioimmunoassay calibration diagram using SHG 1 from 0.1 to 1,000pg was sigmoid-shaped and presented straight line portion from 10 to 100pg on semilogarithmic scale. Reproducibility of determinations on a group of serum samples was studied. The relative standard deviation of the measured values was 8.6% above 100pg/ml and 12.9% below 100pg/ml of serum gastrm concentration. Comparative immunoreactivities of caerulein, pentagastrin, benzyloxycarbonyl tetragastrin in cross reactions versus 125I-SHG was studied. Relative inhibitory potencies, taken at the reduction of B% by 100pg of SHG and normalized to SHG, were as follows: SHG I 1, caerulein 0.0064, pentagastrin 0.00014 and benzyloxycarbonyl tetragastrin 0.0000068 (molar ratio). Fasting serum gastrin levels of the patients of various diseases were measured by this method. The gastrin levels measured were as follows (mean+-standard deviation): control group 202.4+-101.1pg/ml, gastric ulcer 137.7+-100.6 pg/ml, duodenal ulcer 163.0+-98.2 pg/ml, renal insufficiency 763.9+-507.3 pg/ml, liver cirrhosis 197.4+-124.2 pg/ml, diabetes mellitus 177.5+-79.8 pg/ml.
PracticeClinical medicine
Keywords

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