| Japanese |
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| Title | 99mTc-標識赤血球による脾シンチグラフィー |
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| Subtitle | 原著 |
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| Authors | 内田立身, 中島言子*, 刈米重夫 |
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| Organization | 京都大学医学部第一内科, *京大病院中央放射性同位元素診療部 |
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| Journal | 核医学 |
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| Volume | 10 |
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| Number | 2 |
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| Page | 79-89 |
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| Year/Month | 1973/4 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「要約」99mTc-pertechnetate標識赤血球による脾シンチグラフィーを得る目的で, 99mTcによる赤血球の標識法および標識赤血球の熱処理による障害法を検討し, 次に記す方法に標準化した. 1) 被検者血液8mlを, 2ml ACDとともに採血し, 赤血球と血漿に分離する. 2) 赤血球は生理的食塩水で2回洗滌する. 3) 洗滌赤血球に, 99mTc-pertechnetate 0.1〜0.5ml(1〜3mCi)を加え, 37℃で30分間incubateする. 4) 標識赤血球に, reducing agentとして新たに調整したSnCl2・H2O溶液(100μg/ml ACD)0.1mlを加え, 10分間室温に放置する. 5) 標識赤血球を2回生理的食塩水で洗滌し, さきに保存した患者血漿に浮遊する. 6) 49±0.5℃の恒温槽で, 7〜10分間加温しつつ障害する. 7) 冷却後, 被検者の静脈内に投与し, 1時間後よりスキャニングを行なう. 以上の方法でほぼ満足しうるスキャン像を得た. 同時に施行した51Cr熱処理法による脾スキャニング像と比較し, 両者の得失を検討した. 両標識法は, それぞれ一長一短を有し, 症例に応じて核種を使い分けることが望ましいことを述べた. 本論文の要旨は, 第12回日本核医学会総会186席(於・京都, 1972年)で発表した. 脇坂行一教授の御指導に深謝します. |
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| Practice | 臨床医学：一般 |
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| English |
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| Title | Splenic Scintigraphy by 99mTc-labeled Red Cells |
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| Subtitle | Original |
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| Authors | T.UCHIDA, K.NAKAJIMA*, S.KARIYONE |
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| Organization | The First Division, Department of Internal Medicine, Faculty of Medicine, Kyoto University, *Central Radioisotop Clinic, Kyoto Universiy Hospital |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 10 |
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| Number | 2 |
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| Page | 79-89 |
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| Year/Month | 1973/4 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | The method for red cell labeling by 99mTc-pertechnetate and damage of labeled cells in a manner that results in their sequestration by the spleen was evaluated to obtain spleen scanning. The standardized method for red cell labeling and spleen scanning is as follows; 1. Add 8 ml of the patient's blood to a sterile capped tube containing 2 ml ACD. 2. Separate the red cells and plasma by centrifugation and wash the red cells twice with isotonic saline. 3. Add 99mTc-pertechnetate in 0.1-0.5 ml saline (1-3 mCi) to red cells and incubate for 30 minutes at 37℃. 4. Add 0.1 ml of 100μg SnCl2・2H2O/ml ACD, freshly prepard as a reducing agent and stand for 10 minutes at room temperature. 5. Wash twice with isotonic saline and re-suspend in the patient's own plasma. 6. Heat the labeled red cells in a 49+-0.5℃ waterbath for 7-10 minutes, 7. Cool at room temperature and infuse to the patient. The radioactivity of damaged red cells was accumulated to the spleen enough to obtain the suitable scan images. Two methods of 51Cr-heated red cells and 99mTc-labeled cells were compared. No difference was found in spleen size obtained by two techniques. 51Cr-method was characterized by expressing a whole image of the spleen and 99mTc.method by expressing rather superficial or sectional image. Labeling technique of 99mTc-method was complicated and it took two hours through all of the procedure. That of 51Cr-method, on the other hand, was somewhat simple and it took an hour. Splenic radiation was 3 rad with 600 μCi in 99mTc-method, since the radioactivity remained in the spleen for only a few hours. In 51Cr-method, spleen radiation was 5 rad with 200 μCi. |
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| Practice | Clinical medicine |
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