| Japanese |
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| Title | 甲状腺のRI in vitro test (Resin Strip法) [II] Competitive Protein Binding Analysis |
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| Subtitle | 原著 |
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| Authors | 沢崎憲夫, 宮井潔, 片山善章, 熊原雄一 |
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| Organization | 大阪大学医学部 中央臨床検査部 |
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| Journal | 核医学 |
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| Volume | 10 |
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| Number | 1 |
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| Page | 13-15 |
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| Year/Month | 1973/2 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「諸言」血中甲状腺ホルモンのうちthyroxine(T4)の測定法として古くから用いられてきた血漿蛋白結合ヨード(PBI)は, 測定法が煩雑で特異性にも問題が多かった. 1964年Murphyによって開発されたcompetitive protein binding analysisは, 一定量の131I(または125I)標識T4と, これに特異的に結合するthyroxine binding protein(TBP)とを反応させ, この反応系に測定すべき非標識T4を競合させた後, 結合-未結合物を分離し, その放射活性を測定するもので, T4とTBPの特異的な結合を利用したすぐれたT4測定法である. その後T4の抽出方法, 結合-未結合物の分離方法などが種々改良され, 多くの変法が報告されている. そのうちで, 近年Mallinckrodt社によって開発されたRes-O-Mat T4法は, アルコールによってT4を抽出し, 結合-未結合物の分離には特殊なresin stripを用いる方法である. |
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| Practice | 臨床医学：一般 |
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| English |
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| Title | Evaluation of RI in vitro test for thyroid function (resin strip technique) II. Competitive protein binding analysis |
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| Subtitle | Original |
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| Authors | Norio SAWAZAKI, Kiyoshi MYAI, Yoshiaki KATAYAMA, Yuichi KUMAHARA |
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| Organization | Central Laboratory for Clinical Investigation, Osaka University Hospital |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 10 |
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| Number | 1 |
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| Page | 13-15 |
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| Year/Month | 1973/2 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | The resin strip technique(Res-O-Mat T4)as a competitive protein binding analysis for determination of serum thyroxine was evaluated. The extraction efficiency factor reached plateau within 10sec. by ethanol extraction and ranged between 75-80%in normal sera. There was no difference between the extraction efficiency factors in hyperthyroid and hypothyroid sera. The standard deviation of duplicate determinations was 0.5μg/dl(T4 iodine)and the coefficient of variations between assays was 11%. The range of concentration of serum thyroxine iodine(mean+-2 S. D. )was 3.0-7.2μg/dl in euthyroid subjects, 5.8-16.0μg/dl in hyperthyroid patients, 0-3.6μg/dl in hypothyroid patients. Oral administration of 6tablets of Telepaque resulted in a significant elevation of PBI but had no effect on T4levels which were determined by Res-O-Mat T4 methcd. |
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| Practice | Clinical medicine |
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