| English |
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| Title | Radioimmunoassay Past, Present, and Future |
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| Subtitle | |
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| Authors | Rosalyn S.Yalow, Solomon A.Berson |
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| Organization | Radioisotope Service, Veterans Administration Hospital, Department of Medicine, Mt. Sinai School of Medicine of The City University of New York |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 9 |
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| Number | 3 |
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| Page | 287-301 |
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| Year/Month | 1972/6 |
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| Article | Report |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | Radioimmunoassay developed originally out of investigations on the metabolism of insulin. Using 131I-insulin to study insulin turnover in normal and diabetic subjects we made the unexpected observation that the labeled insulin disappeared from the blood stream at a much slower rate in subjects who had been treated with insulin for more than a month than in untreated subjects or those who had been treated for only 2-3 weeks. This difference in behavior of the labeled insulin was unlabeled to the presence or diabetes mellitus, a slow disappearance being observed also in non-diabetic schizophrenic patients undergoing insulin shock therapy. When the plasma radioactivity was characterized by its behavior in 23% Na2SO4, on paper electrophoresis and chromatoelectrophoresis, and on ultracentrifugaton, it appeared that the labeled insulin was bound to a γ globulin in the insulin-treated subjects and was thereby protected from the rapid degradation observed in the control subjects in whom the hormone remained in the free, unbound state. |
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| Practice | Clinical medicine |
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| Keywords | |
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