English
TitleRadioimmunoassay Past, Present, and Future
Subtitle
AuthorsRosalyn S.Yalow, Solomon A.Berson
Authors(kana)
OrganizationRadioisotope Service, Veterans Administration Hospital, Department of Medicine, Mt. Sinai School of Medicine of The City University of New York
JournalThe Japanese Journal of nuclear medicine
Volume9
Number3
Page287-301
Year/Month1972/6
ArticleReport
PublisherTHE JAPANESE SOCIETY OF NUCLEAR MEDICINE
AbstractRadioimmunoassay developed originally out of investigations on the metabolism of insulin. Using 131I-insulin to study insulin turnover in normal and diabetic subjects we made the unexpected observation that the labeled insulin disappeared from the blood stream at a much slower rate in subjects who had been treated with insulin for more than a month than in untreated subjects or those who had been treated for only 2-3 weeks. This difference in behavior of the labeled insulin was unlabeled to the presence or diabetes mellitus, a slow disappearance being observed also in non-diabetic schizophrenic patients undergoing insulin shock therapy. When the plasma radioactivity was characterized by its behavior in 23% Na2SO4, on paper electrophoresis and chromatoelectrophoresis, and on ultracentrifugaton, it appeared that the labeled insulin was bound to a globulin in the insulin-treated subjects and was thereby protected from the rapid degradation observed in the control subjects in whom the hormone remained in the free, unbound state.
PracticeClinical medicine
Keywords

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